•Faecal cortisol metabolites (FCM) were measured after ACTH injection and handling.•ACTH induced a strong adrenocortical activation and handling an intermediate response.•Unexpectedly, baseline FCM levels before handling were higher in more confident foxes.•We suggest a 4h delay to first faeces collection, then sampling every 3rdh for 12h.•Our study confirms FCM as a valid indicator of adrenocortical activity in silver foxes.
Applied Animal Behaviour Science – Elsevier
Published: Dec 1, 2017
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