Expression of recombinant SnRK1 in E. coli. Characterization of adenine nucleotide binding to the SnRK1.1/AKINβγ-β3 complex

Expression of recombinant SnRK1 in E. coli. Characterization of adenine nucleotide binding to the... Plant Science 263 (2017) 116–125 Contents lists available at ScienceDirect Plant Science journal homepage: www.elsevier.com/locate/plantsci Expression of recombinant SnRK1 in E. coli. Characterization of adenine MARK nucleotide binding to the SnRK1.1/AKINβγ-β3 complex a a a a José Luis Maya-Bernal , Alejandra Ávila , Ana Ruiz-Gayosso , Ricardo Trejo-Fregoso , c b a a Nancy Pulido , Alejandro Sosa-Peinado , Esther Zúñiga-Sánchez , Eleazar Martínez-Barajas , a a, Rogelio Rodríguez-Sotres , Patricia Coello Departamento de Bioquímica, Facultad de Química, UNAM, Ciudad de México 04510, Mexico Departamento de Bioquímica, Facultad de Medicina, UNAM, Ciudad de México 04510, Mexico Centro de Investigaciones Químicas, UAEM, Morelos, 62210, Mexico ARTICLE I NFO ABSTRACT Keywords: The SnRK1 complexes in plants belong to the family of AMPK/SNF1 kinases, which have been associated with Arabidopsis thaliana the control of energy balance, in addition to being involved in the regulation of other aspects of plant growth and CBS motifs development. Analysis of complex formation indicates that increased activity is achieved when the catalytic Nucleotide binding subunit is phosphorylated and bound to regulatory subunits. SnRK1.1 subunit activity is higher than that of SnRK1 complexes SnRK1.2, which also exhibits reduced activation due to the regulatory subunits. The catalytic phosphomimetic Phosphorylation http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Science Elsevier

Expression of recombinant SnRK1 in E. coli. Characterization of adenine nucleotide binding to the SnRK1.1/AKINβγ-β3 complex

Loading next page...
 
/lp/elsevier/expression-of-recombinant-snrk1-in-e-coli-characterization-of-adenine-J8pVg07Hp2
Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0168-9452
D.O.I.
10.1016/j.plantsci.2017.07.005
Publisher site
See Article on Publisher Site

Abstract

Plant Science 263 (2017) 116–125 Contents lists available at ScienceDirect Plant Science journal homepage: www.elsevier.com/locate/plantsci Expression of recombinant SnRK1 in E. coli. Characterization of adenine MARK nucleotide binding to the SnRK1.1/AKINβγ-β3 complex a a a a José Luis Maya-Bernal , Alejandra Ávila , Ana Ruiz-Gayosso , Ricardo Trejo-Fregoso , c b a a Nancy Pulido , Alejandro Sosa-Peinado , Esther Zúñiga-Sánchez , Eleazar Martínez-Barajas , a a, Rogelio Rodríguez-Sotres , Patricia Coello Departamento de Bioquímica, Facultad de Química, UNAM, Ciudad de México 04510, Mexico Departamento de Bioquímica, Facultad de Medicina, UNAM, Ciudad de México 04510, Mexico Centro de Investigaciones Químicas, UAEM, Morelos, 62210, Mexico ARTICLE I NFO ABSTRACT Keywords: The SnRK1 complexes in plants belong to the family of AMPK/SNF1 kinases, which have been associated with Arabidopsis thaliana the control of energy balance, in addition to being involved in the regulation of other aspects of plant growth and CBS motifs development. Analysis of complex formation indicates that increased activity is achieved when the catalytic Nucleotide binding subunit is phosphorylated and bound to regulatory subunits. SnRK1.1 subunit activity is higher than that of SnRK1 complexes SnRK1.2, which also exhibits reduced activation due to the regulatory subunits. The catalytic phosphomimetic Phosphorylation

Journal

Plant ScienceElsevier

Published: Oct 1, 2017

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off