Expression of endothelial nitric oxide synthase in the ischemic penumbra: relationship to expression of neuronal nitric oxide synthase and vascular endothelial growth factor

Expression of endothelial nitric oxide synthase in the ischemic penumbra: relationship to... Expressional patterns of the endothelial and neuronal forms of nitric oxide synthase (NOS) in cerebral ischemia were studied utilizing a permanent middle cerebral artery occlusion (PMCAO) model. Motor performance and infarct volumes were determined in the rats. Immunohistochemical staining for eNOS, nNOS and neurofilament were performed at 1, 2, 3, 5, 7 and 14 days after PMCAO. Vascular endothelial growth factor (VEGF) expression was determined by in-situ hybridization. PMCAO caused a reproducible cortical infarct with motor deficits in the rats. Double immunohistochemical stainings indicated that eNOS and nNOS were induced in ischemic neurons. Most stained neurons were positive for both NOS forms but some reacted with only one NOS antibody. nNOS expression peaked at 24–48 h after PMCAO, stained mainly the cytoplasm of core neurons, and disappeared after the 3rd day. eNOS expression increased until the 7th day, stained mainly the cytoplasm and membrane of penumbral cells and disappeared by the 14th day after PMCAO. VEGF expression was significantly induced in the penumbral zone in a similar distribution to eNOS. The anatomical and temporal pattern of VEGF and eNOS induction in the brain after permanent ischemia suggest that these mediators may play a role in protecting penumbral tissue from additional ischemic damage. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Brain Research Elsevier

Expression of endothelial nitric oxide synthase in the ischemic penumbra: relationship to expression of neuronal nitric oxide synthase and vascular endothelial growth factor

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Publisher
Elsevier
Copyright
Copyright © 2001 Elsevier Science B.V.
ISSN
0006-8993
D.O.I.
10.1016/S0006-8993(01)02561-6
Publisher site
See Article on Publisher Site

Abstract

Expressional patterns of the endothelial and neuronal forms of nitric oxide synthase (NOS) in cerebral ischemia were studied utilizing a permanent middle cerebral artery occlusion (PMCAO) model. Motor performance and infarct volumes were determined in the rats. Immunohistochemical staining for eNOS, nNOS and neurofilament were performed at 1, 2, 3, 5, 7 and 14 days after PMCAO. Vascular endothelial growth factor (VEGF) expression was determined by in-situ hybridization. PMCAO caused a reproducible cortical infarct with motor deficits in the rats. Double immunohistochemical stainings indicated that eNOS and nNOS were induced in ischemic neurons. Most stained neurons were positive for both NOS forms but some reacted with only one NOS antibody. nNOS expression peaked at 24–48 h after PMCAO, stained mainly the cytoplasm of core neurons, and disappeared after the 3rd day. eNOS expression increased until the 7th day, stained mainly the cytoplasm and membrane of penumbral cells and disappeared by the 14th day after PMCAO. VEGF expression was significantly induced in the penumbral zone in a similar distribution to eNOS. The anatomical and temporal pattern of VEGF and eNOS induction in the brain after permanent ischemia suggest that these mediators may play a role in protecting penumbral tissue from additional ischemic damage.

Journal

Brain ResearchElsevier

Published: Aug 3, 2001

References

  • The complex role of nitric oxide in the pathophysiology of focal cerebral ischemia
    Dalkara, T.; Moskowitz, M.A.
  • Regulation of the cerebral circulation: role of endothelium and potassium channels
    Faraci, F.M.; Heistad, D.D.
  • VEGF upregulates ecNOS message, protein, and NO production in human endothelial cells
    Hood, J.D.; Meininger, C.J.; Ziche, M.; Granger, H.J.
  • Nitric oxide modulates microvascular permeability
    Kubes, P.; Granger, D.N.
  • The anti-aggregating properties of vascular endothelium: interactions between prostacyclin and nitric oxide
    Radomski, M.W.; Palmer, R.M.; Moncada, S.
  • Inhibition of endothelial cell proliferation and bFGF-induced phenotypic modulation by nitric oxide
    RayChaudhury, A.; Frischer, H.; Malik, A.B.
  • Differential expression of neuronal and endothelial nitric oxide synthase in blood vessels of the rat brain
    Seidel, B.; Stanarius, A.; Wolf, G.
  • The role of nitric oxide in the pathophysiology of thromboembolic stroke in the rat
    Stagliano, N.E.; Dietrich, W.D.; Prado, R.; Green, E.J.; Busto, R.
  • Distribution of the endothelial constitutive nitric oxide synthase in the developing rat brain: an immunohistochemical study
    Topel, I.; Stanarius, A.; Wolf, G.

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