Expansion of the Genetic Code Enables Design of a Novel “Gold” Class of Green Fluorescent Proteins

Expansion of the Genetic Code Enables Design of a Novel “Gold” Class of Green Fluorescent... Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora ( av ). These approaches have been based on classical engineering with the 20 canonical amino acids. We report here an expansion of these efforts by incorporation of an amino substituted variant of tryptophan into the “cyan” GFP mutant, which turned it into a “gold” variant. This variant possesses a red shift in emission unprecedented for any av FP, similar to “red” FPs, but with enhanced stability and a very low aggregation tendency. An increasing number of non-natural amino acids are available for chromophore redesign (by engineering of the genetic code) and enable new general strategies to generate novel classes of tailor-made GFP proteins. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Molecular Biology Elsevier

Expansion of the Genetic Code Enables Design of a Novel “Gold” Class of Green Fluorescent Proteins

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Publisher
Elsevier
Copyright
Copyright © 2003 Elsevier Science Ltd
ISSN
0022-2836
D.O.I.
10.1016/S0022-2836(03)00364-4
Publisher site
See Article on Publisher Site

Abstract

Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora ( av ). These approaches have been based on classical engineering with the 20 canonical amino acids. We report here an expansion of these efforts by incorporation of an amino substituted variant of tryptophan into the “cyan” GFP mutant, which turned it into a “gold” variant. This variant possesses a red shift in emission unprecedented for any av FP, similar to “red” FPs, but with enhanced stability and a very low aggregation tendency. An increasing number of non-natural amino acids are available for chromophore redesign (by engineering of the genetic code) and enable new general strategies to generate novel classes of tailor-made GFP proteins.

Journal

Journal of Molecular BiologyElsevier

Published: May 16, 2003

References

  • Structure and dynamics of green fluorescent protein
    Phillips, G.N.
  • Towards new protein engineering: in vivo building and folding of protein shuttles for drug delivery and targeting by the selective pressure incorporation (SPI) method
    Minks, C.; Alefelder, S.; Moroder, L.; Huber, R.; Budisa, N.

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