Evaluation of a biomimetic 3D substrate based on the Human Elastin-like Polypeptides (HELPs) model system for elastolytic activity detection

Evaluation of a biomimetic 3D substrate based on the Human Elastin-like Polypeptides (HELPs)... •Recombinantly expressed human elastin-like polypeptides are assessed as substrates for elastolytic activity detection.•The elastin biomimetic 3D matrix is degraded by P. aeruginosa exoproteases.•Matrix degradation results in the release of a dye that can be easily measured by spectrophotometric reading.•The biomimetic system we set up represents a relevant in vitro model that well resembles the features of the intact tissue elastin. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biotechnology Elsevier

Evaluation of a biomimetic 3D substrate based on the Human Elastin-like Polypeptides (HELPs) model system for elastolytic activity detection

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0168-1656
eISSN
1873-4863
D.O.I.
10.1016/j.jbiotec.2017.06.006
Publisher site
See Article on Publisher Site

Abstract

•Recombinantly expressed human elastin-like polypeptides are assessed as substrates for elastolytic activity detection.•The elastin biomimetic 3D matrix is degraded by P. aeruginosa exoproteases.•Matrix degradation results in the release of a dye that can be easily measured by spectrophotometric reading.•The biomimetic system we set up represents a relevant in vitro model that well resembles the features of the intact tissue elastin.

Journal

Journal of BiotechnologyElsevier

Published: Aug 10, 2017

References

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