Acta Biomaterialia 71 (2018) 330–338 Contents lists available at ScienceDirect Acta Biomaterialia journal homepage: www.elsevier.com/locate/actabiomat Full length article a,b c a,b a,b,⇑ Bo Huang , Walter L. Siqueira , Dennis G. Cvitkovitch , Yoav Finer Faculty of Dentistry, University of Toronto, Ontario, Canada Institute of Biomaterials and Biomedical Engineering, University of Toronto, Canada School of Dentistry and Department of Biochemistry, Schulich School of Medicine & Dentistry, Western University, Canada ar ti c l e i nf o ab stra ct Article history: Objectives: To identify and characterize speciﬁc esterases from S. mutans with degradative activity Received 31 October 2017 toward methacrylate-based resin monomers. Received in revised form 16 February 2018 Methods: Out of several putative esterases, an esterase encoded in an Open Reading Frame as SMU_118c Accepted 20 February 2018 (The National Center for Biotechnology Information, NCBI), was found to have true hydrolase activities. Available online 1 March 2018 SMU_118c was cloned, expressed, puriﬁed and further characterized for its respective hydrolytic activity towards ester-containing nitrophenyl substrates and the universal resin monomers bis-phenyl-glycidyl- Keywords: dimethacrylate (bisGMA) and triethyleneglycol dimethacrylate (TEGDMA) at neutral (7.0) or cariogenic Streptococcus mutans (5.5) pH. Mass spectrometry (MS) was used to verify the expression of SMU_118c
Acta Biomaterialia – Elsevier
Published: Apr 15, 2018
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