Establishment of an efficient Agrobacterium-mediated genetic transformation method in Macleaya cordata

Establishment of an efficient Agrobacterium-mediated genetic transformation method in Macleaya... •This is the first report of Agrobacterium-mediated transformation of M. cordata.•The transformation efficiency was influenced by different type of explants.•The combination of vacuum infiltration 10min, 75mg−1 kanamycin and 400mgl−1 timentin obtained the highest transformation rate.•The protocol developed in the present study would be useful for M. cordata genetic breeding and improvement. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Scientia Horticulturae Elsevier

Establishment of an efficient Agrobacterium-mediated genetic transformation method in Macleaya cordata

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Ltd
ISSN
0304-4238
eISSN
1879-1018
D.O.I.
10.1016/j.scienta.2017.09.004
Publisher site
See Article on Publisher Site

Abstract

•This is the first report of Agrobacterium-mediated transformation of M. cordata.•The transformation efficiency was influenced by different type of explants.•The combination of vacuum infiltration 10min, 75mg−1 kanamycin and 400mgl−1 timentin obtained the highest transformation rate.•The protocol developed in the present study would be useful for M. cordata genetic breeding and improvement.

Journal

Scientia HorticulturaeElsevier

Published: Dec 19, 2017

References

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