Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes

Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in... Background : Resistin is a recently identified adipocyte-secreted hormone in rodents, and has been proposed to serve as a link between obesity and insulin resistance. The aim of this study was to develop a sensitive enzyme-linked immunosorbent assay (ELISA) for human resistin and evaluate serum resistin concentrations in normal subjects and patients with type 2 diabetes. Methods : Using ELISA developed by two polyclonal antibodies, resistin concentrations were measured in 90 patients with type 2 diabetes and compared to 74 healthy control subjects. Results : This ELISA has high specificity and sensitivity over the concentration of range 0.5–100 ng/ml with good percentage recovery (97.1±4.7%) and reproducibility (within-day assay, CV=4.8–8.6%; between-day assay, CV=5.6–9.7%). The mean concentration of resistin in sera from type 2 diabetic patients was significantly higher than that in normal subjects (mean±S.E.: 20.8±0.7 vs. 14.9±0.5 ng/ml, p <0.001). A moderate positive correlation was observed between serum resistin levels and body mass indices in both normal subjects ( r =0.412, p <0.0003) and patients with type 2 diabetes ( r =0.395, p <0.0001). Conclusions : Our ELISA will be useful to confirm the physiological and pathophysiological role of resistin in humans. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinica Chimica Acta Elsevier

Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes

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Abstract

Background : Resistin is a recently identified adipocyte-secreted hormone in rodents, and has been proposed to serve as a link between obesity and insulin resistance. The aim of this study was to develop a sensitive enzyme-linked immunosorbent assay (ELISA) for human resistin and evaluate serum resistin concentrations in normal subjects and patients with type 2 diabetes. Methods : Using ELISA developed by two polyclonal antibodies, resistin concentrations were measured in 90 patients with type 2 diabetes and compared to 74 healthy control subjects. Results : This ELISA has high specificity and sensitivity over the concentration of range 0.5–100 ng/ml with good percentage recovery (97.1±4.7%) and reproducibility (within-day assay, CV=4.8–8.6%; between-day assay, CV=5.6–9.7%). The mean concentration of resistin in sera from type 2 diabetic patients was significantly higher than that in normal subjects (mean±S.E.: 20.8±0.7 vs. 14.9±0.5 ng/ml, p <0.001). A moderate positive correlation was observed between serum resistin levels and body mass indices in both normal subjects ( r =0.412, p <0.0003) and patients with type 2 diabetes ( r =0.395, p <0.0001). Conclusions : Our ELISA will be useful to confirm the physiological and pathophysiological role of resistin in humans.

Journal

Clinica Chimica ActaElsevier

Published: Jan 1, 2004

References

  • Obesity and insulin resistance
    Kahn, B.B.; Flier, J.S.
  • Insulin resistance and type 2 diabetes are not related to resistin expression in human fat cells or skeletal muscle
    Nagaev, I.; Smith, U.

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