Enzyme activity of horseradish peroxidase in surfactant-free microemulsions

Enzyme activity of horseradish peroxidase in surfactant-free microemulsions Journal of Colloid and Interface Science 516 (2018) 466–475 Contents lists available at ScienceDirect Journal of Colloid and Interface Science journal homepage: www.elsevier.com/locate/jcis Regular Article Enzyme activity of horseradish peroxidase in surfactant-free microemulsions a a b c a,⇑ Sebastian Krickl , Didier Touraud , Pierre Bauduin , Thomas Zinn , Werner Kunz Institute of Physical and Theoretical Chemistry, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany Institut de Chimie Séparative de Marcoule (ICSM), UMR 5257 (CEA, CNRS, UM, ENSCM), BP 17171, 30207 Bagnols-sur-Cèze, France European Synchrotron Radiation Facility (ESRF), 38043 Grenoble Cedex 9, France graphical a bstract ar ti c l e i nf o ab stra ct Article history: In the present contribution, we investigated the influence of the structuring of surfactant-free Received 15 December 2017 microemulsions (SFME) (water/1-propanol/limonene and water/tert-butanol/limonene) on the enzyme Revised 22 January 2018 activity of horseradish peroxidase (HRP). To this purpose, the oxidation of 2,2 -azino-bis(3-ethylbenzo Accepted 22 January 2018 thiazoline-6-sulfonic acid) diammonium salt (ABTS) with hydrogen peroxide was chosen as a model reac- Available online 6 February 2018 tion. Enzymatic activities in SFMEs of varying compositions were investigated by UV–Vis spectroscopy and compared to the enzyme activity in pure buffer solution. Dynamic http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Colloid and Interface Science Elsevier

Enzyme activity of horseradish peroxidase in surfactant-free microemulsions

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier Inc.
ISSN
0021-9797
eISSN
1095-7103
D.O.I.
10.1016/j.jcis.2018.01.077
Publisher site
See Article on Publisher Site

Abstract

Journal of Colloid and Interface Science 516 (2018) 466–475 Contents lists available at ScienceDirect Journal of Colloid and Interface Science journal homepage: www.elsevier.com/locate/jcis Regular Article Enzyme activity of horseradish peroxidase in surfactant-free microemulsions a a b c a,⇑ Sebastian Krickl , Didier Touraud , Pierre Bauduin , Thomas Zinn , Werner Kunz Institute of Physical and Theoretical Chemistry, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany Institut de Chimie Séparative de Marcoule (ICSM), UMR 5257 (CEA, CNRS, UM, ENSCM), BP 17171, 30207 Bagnols-sur-Cèze, France European Synchrotron Radiation Facility (ESRF), 38043 Grenoble Cedex 9, France graphical a bstract ar ti c l e i nf o ab stra ct Article history: In the present contribution, we investigated the influence of the structuring of surfactant-free Received 15 December 2017 microemulsions (SFME) (water/1-propanol/limonene and water/tert-butanol/limonene) on the enzyme Revised 22 January 2018 activity of horseradish peroxidase (HRP). To this purpose, the oxidation of 2,2 -azino-bis(3-ethylbenzo Accepted 22 January 2018 thiazoline-6-sulfonic acid) diammonium salt (ABTS) with hydrogen peroxide was chosen as a model reac- Available online 6 February 2018 tion. Enzymatic activities in SFMEs of varying compositions were investigated by UV–Vis spectroscopy and compared to the enzyme activity in pure buffer solution. Dynamic

Journal

Journal of Colloid and Interface ScienceElsevier

Published: Apr 15, 2018

References

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