Bioresource Technology 249 (2018) 9–15 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech Engineering Pediococcus acidilactici with xylose assimilation pathway for high titer cellulosic L-lactic acid fermentation Zhongyang Qiu, Qiuqiang Gao, Jie Bao State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China GR APHICAL A BSTRACT ARTICLE I NFO ABSTRACT Keywords: Xylose-assimilating pathways were constructed in the parental Pediococcus acidilactici strain and evolutionarily Pediococcus acidilactici adapted to yield a highly stable co-fermentation strain for L-lactic acid production. The phosphoketolase L-Lactic acid pathway (PK) was blocked for reduction of acetic acid generation by disrupting phosphoketolase (pkt) gene. The Xylose-assimilating pathway pentose phosphate pathway (PPP) was reconstructed for xylose assimilation by integrating four heterologous Adaptive evolution genes encoding transketolase (tkt), transaldolase (tal), xylose isomerase (xylA) and xylulokinase (xylB) into the P. Lignocellulose acidilactici chromosome. The xylose-assimilating ability of the constructed strain was signiﬁcantly improved by long term adaptive evolution. The engineered strain was applied to the simultaneous sacchariﬁcation and co- fermentation (SSCF) under high solids loading of wheat straw. The L-lactic acid titer, productivity and xylose conversion reached the record high at 130.8 ± 1.6 g/L, 1.82 ± 0.0
Bioresource Technology – Elsevier
Published: Feb 1, 2018
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