Emerging mechanisms of eukaryotic DNA replication initiation

Emerging mechanisms of eukaryotic DNA replication initiation Recent research has focused on proteins important for early steps in replication in eukaryotes, and particularly on Cdc6/Cdc18, the MCMs, and Cdc45. Although it is still unclear exactly what role these proteins play, it is possible that they are analogous to initiation proteins in prokaryotes. One specific model is that MCMs form a hexameric helicase at replication forks, and Cdc6/Cdc18 acts as a ‘clamp-loader’ required to lock the MCMs around DNA. The MCMs appear to be the target of Cdc7-Dbf4 kinase acting at individual replication origins. Finally, Cdc45 interacts with MCMs and may shed light on how cyclin-dependent kinases activate DNA replication. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Opinion in Cell Biology Elsevier

Emerging mechanisms of eukaryotic DNA replication initiation

Current Opinion in Cell Biology, Volume 10 (6) – Dec 1, 1998

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Publisher
Elsevier
Copyright
Copyright © 1998 Elsevier Ltd
ISSN
0955-0674
D.O.I.
10.1016/S0955-0674(98)80117-8
Publisher site
See Article on Publisher Site

Abstract

Recent research has focused on proteins important for early steps in replication in eukaryotes, and particularly on Cdc6/Cdc18, the MCMs, and Cdc45. Although it is still unclear exactly what role these proteins play, it is possible that they are analogous to initiation proteins in prokaryotes. One specific model is that MCMs form a hexameric helicase at replication forks, and Cdc6/Cdc18 acts as a ‘clamp-loader’ required to lock the MCMs around DNA. The MCMs appear to be the target of Cdc7-Dbf4 kinase acting at individual replication origins. Finally, Cdc45 interacts with MCMs and may shed light on how cyclin-dependent kinases activate DNA replication.

Journal

Current Opinion in Cell BiologyElsevier

Published: Dec 1, 1998

References

  • Initiation of DNA replication in eukaryotic cells
    Dutta, A; Bell, SP
  • Replication initiation point mapping
    Gerbi, SA; Bielinsky, AK
  • Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22
    Ishiai, M; Dean, FB; Okumura, K; Abe, M; Moon, KY; Amin, AA; Kagotani, K; Taguchi, H; Murakami, Y; Hanaoka, F
  • The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin
    Romanowski, P; Madine, MA; Rowles, A; Blow, JJ; Laskey, RA
  • Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc6, but dependent on cdk2
    Hua, XH; Newport, J
  • A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast
    Adachi, Y; Usukura, J; Yanagida, M
  • Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7
    Sato, N; Arai, K; Masai, H
  • Cyclin-dependent kinase and initiation at eukaryotic origins: a replication switch?
    Jallepalli, PV; Kelly, TJ
  • The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast
    Drury, LS; Perkins, G; Diffley, JF
  • Identification of Cdc45p, an essential factor required for DNA replication
    Hardy, CF
  • Identification of sna41 gene, which is the suppressor of nda4 mutation and is involved in DNA replication in Schizosaccharomyces pombe
    Miyake, S; Yamashita, S
  • Molecular cloning of a gene required for DNA replication in Ustilago maydis
    Onel, K; Holloman, WK

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