Chemical Engineering Journal 336 (2018) 456–464 Contents lists available at ScienceDirect Chemical Engineering Journal journal homepage: www.elsevier.com/locate/cej Electrospun nanoﬁbrous membranes containing epoxy groups and hydrophilic polyethylene oxide chain for highly active and stable covalent immobilization of lipase ⁎ ⁎ a,b,1, a,1, a a a Xinhua Liu , Yinchun Fang , Xu Yang , Yong Li , Cuie Wang College of Textiles and Clothing, Anhui Polytechnic University, Wuhu 241000, China Technology Public Service Platform for Textile Industry Anhui Province, Wuhu 241000, China GR APHICAL A BSTRACT ARTICLE I NFO ABSTRACT Keywords: Immobilization of enzymes on nanoﬁbrous membranes could oﬀer easy recycling and feasible continuous op- Nanoﬁbrous membranes erations. In this study, a novel terpolymer poly (glycidyl methacrylate-co-methylacrylate)-g-polyethylene oxide Enzyme immobilization (P(GMA-co-MA)-g-PEO) containing reactive epoxy groups and hydrophilic polyethylene oxide branch chain was Electrospinning synthesized. Electrospun P(GMA-co-MA)-g-PEO nanoﬁbrous membrane was used for the immobilization of li- Covalent binding pase molecules by covalent binding with the epoxy groups. The inﬂuences of the enzyme loading and activity by Lipase nanoﬁbrous membranes with diﬀerent content of monomers and immobilization temperature were investigated. The immobilized lipase achieved high enzyme loading of 150 mg/g and the maximum activity of 0.673 U/mg under the optimum immobilization conditions.
Chemical Engineering Journal – Elsevier
Published: Mar 15, 2018
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