Electrospun nanofibrous membranes containing epoxy groups and hydrophilic polyethylene oxide chain for highly active and stable covalent immobilization of lipase

Electrospun nanofibrous membranes containing epoxy groups and hydrophilic polyethylene oxide... Chemical Engineering Journal 336 (2018) 456–464 Contents lists available at ScienceDirect Chemical Engineering Journal journal homepage: www.elsevier.com/locate/cej Electrospun nanofibrous membranes containing epoxy groups and hydrophilic polyethylene oxide chain for highly active and stable covalent immobilization of lipase ⁎ ⁎ a,b,1, a,1, a a a Xinhua Liu , Yinchun Fang , Xu Yang , Yong Li , Cuie Wang College of Textiles and Clothing, Anhui Polytechnic University, Wuhu 241000, China Technology Public Service Platform for Textile Industry Anhui Province, Wuhu 241000, China GR APHICAL A BSTRACT ARTICLE I NFO ABSTRACT Keywords: Immobilization of enzymes on nanofibrous membranes could offer easy recycling and feasible continuous op- Nanofibrous membranes erations. In this study, a novel terpolymer poly (glycidyl methacrylate-co-methylacrylate)-g-polyethylene oxide Enzyme immobilization (P(GMA-co-MA)-g-PEO) containing reactive epoxy groups and hydrophilic polyethylene oxide branch chain was Electrospinning synthesized. Electrospun P(GMA-co-MA)-g-PEO nanofibrous membrane was used for the immobilization of li- Covalent binding pase molecules by covalent binding with the epoxy groups. The influences of the enzyme loading and activity by Lipase nanofibrous membranes with different content of monomers and immobilization temperature were investigated. The immobilized lipase achieved high enzyme loading of 150 mg/g and the maximum activity of 0.673 U/mg under the optimum immobilization conditions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chemical Engineering Journal Elsevier

Electrospun nanofibrous membranes containing epoxy groups and hydrophilic polyethylene oxide chain for highly active and stable covalent immobilization of lipase

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
1385-8947
eISSN
1873-3212
D.O.I.
10.1016/j.cej.2017.12.048
Publisher site
See Article on Publisher Site

Abstract

Chemical Engineering Journal 336 (2018) 456–464 Contents lists available at ScienceDirect Chemical Engineering Journal journal homepage: www.elsevier.com/locate/cej Electrospun nanofibrous membranes containing epoxy groups and hydrophilic polyethylene oxide chain for highly active and stable covalent immobilization of lipase ⁎ ⁎ a,b,1, a,1, a a a Xinhua Liu , Yinchun Fang , Xu Yang , Yong Li , Cuie Wang College of Textiles and Clothing, Anhui Polytechnic University, Wuhu 241000, China Technology Public Service Platform for Textile Industry Anhui Province, Wuhu 241000, China GR APHICAL A BSTRACT ARTICLE I NFO ABSTRACT Keywords: Immobilization of enzymes on nanofibrous membranes could offer easy recycling and feasible continuous op- Nanofibrous membranes erations. In this study, a novel terpolymer poly (glycidyl methacrylate-co-methylacrylate)-g-polyethylene oxide Enzyme immobilization (P(GMA-co-MA)-g-PEO) containing reactive epoxy groups and hydrophilic polyethylene oxide branch chain was Electrospinning synthesized. Electrospun P(GMA-co-MA)-g-PEO nanofibrous membrane was used for the immobilization of li- Covalent binding pase molecules by covalent binding with the epoxy groups. The influences of the enzyme loading and activity by Lipase nanofibrous membranes with different content of monomers and immobilization temperature were investigated. The immobilized lipase achieved high enzyme loading of 150 mg/g and the maximum activity of 0.673 U/mg under the optimum immobilization conditions.

Journal

Chemical Engineering JournalElsevier

Published: Mar 15, 2018

References

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