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Efficient Agrobacterium -mediated transformation and recovery of transgenic fig ( Ficus carica L.) plants

Efficient Agrobacterium -mediated transformation and recovery of transgenic fig ( Ficus carica... An efficient and reproducible system for regeneration and Agrobacterium -mediated transformation of the common fig ( Ficus carica L.) cultivars Brown Turkey (fresh consumption) and Smyrna (dry consumption) was developed. Optimal shoot regeneration (up to 100%) was obtained on MS basal salt mixture supplemented with 100 mg l −1 myo-inositol, 1 mg l −1 thiamine HCl and addition of 2.0 mg l −1 thidiazuron (TDZ), 2 mg l −1 indole-3-butyric acid (IBA), 4% sucrose and 0.8% agar. Regeneration was highly dependent on the dorsoventral orientation of the explants. When explants were cultured with the adaxial surface up, 100% regeneration was achieved with more than five shoots per regenerating explant in both studied cultivars. In contrast, if leaves were placed with their abaxial side up, shoot regeneration took place, but still mostly from the adaxial surface. Leaf explants of in vitro propagated plants were co-cultivated with the disarmed Agrobacterium strain EHA105 harboring the plasmid pME504 that carried the uid A - intron and npt II genes. Transformation efficiencies were in a range of 1.7–10.0% for cv. Brown Turkey and 2.8–7.8% for Smyrna. The transgenic nature of the regenerated plants was confirmed by molecular analyses (PCR and Southern blot) as well as by GUS staining. Similar to regeneration, the orientation of the leaf surface during organogenesis was a key factor for successful transformation. Successful transformation of commercial fig cultivars provides a new promising tool for the introduction of foreign genes into transgenic fig cultivars. The regeneration and transformation methodologies described here may pave the way for transgenic varieties with improved agronomic characteristics, such as storability and disease resistance, and will provide a means for the production of foreign proteins in the edible parts of fig, leading to improved nutritional and/or pharmaceutical composition. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Science Elsevier

Efficient Agrobacterium -mediated transformation and recovery of transgenic fig ( Ficus carica L.) plants

Publisher
Elsevier
Copyright
Copyright © 2004 Elsevier Ireland Ltd
ISSN
0168-9452
D.O.I.
10.1016/j.plantsci.2004.12.007
Publisher site
See Article on Publisher Site

References

  • Plant regeneration from axillary buds of a mature tree of Ficus religiosa
    Deshpande, S.R.; Josekutty, P.C.; Prathapasenan, G.
  • In vitro plant regeneration of fig ( Ficus carica L. cv. gular) using apical buds from mature trees
    Kumar, V.; Radha, A.; Kumar Chitta, S.
  • Exogenous phytohormone-independent growth and regeneration of tobacco plants transgenic for the 6b gene of Agrobacterium tumefaciens AKE10
    Wabiko, H.; Minemura, M.
  • Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot
    Perez-Tornero, O.; Egea, J.; Vanoostende, A.; Burgos, L.
  • Auxin type and timing of application determine the activation of the developmental program during in vitro organogenesis in apple
    Yancheva, S.D.; Golubowicz, S.; Fisher, E.; Lev-Yadun, S.; Flaishman, M.A.

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