Effects of novelty and pain on behavior and hippocampal extracellular ACh levels in male and female rats

Effects of novelty and pain on behavior and hippocampal extracellular ACh levels in male and... In vivo microdialysis was used to assess the effects of novelty and pain on hippocampal ACh release in male and female rats. Experiments were carried out during the dark phase and consisted of 2 days of tests: on Day 1, after Baseline 1, animals were exposed to a new cage (Novelty) to which, 30 min later, a plastic cylinder (Object) was introduced. On Day 2, after Baseline 2, the Formalin test (50 μl of formalin 10%, s.c. injected in the dorsal hindpaw) was carried out in the animal's home cage. All behaviors were recorded. The extracellular levels of ACh in the dorsal hippocampus were estimated, in 10-min samples, by assay of ACh in the dialysates by HPLC. On Day 1 the raw values of ACh were higher in females than in males, but no sex difference was present when the percentage of change was considered. In both sexes the Novelty and Object tests induced an increase in ACh levels with respect to Baseline. Higher levels of exploration were present in females than males during the first 10 min of Novelty. On Day 2, ACh release increased in both sexes during the Formalin test. No sex difference in either ACh raw values or the percentages of change were found. Females showed higher levels of licking and lower levels of activity than males. The present study shows that novelty and pain induce similar hippocampal cholinergic activation in male and female rats but different behaviors. The results are discussed in light of the several anatomical and functional sex differences present in the hippocampus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Brain Research Elsevier

Effects of novelty and pain on behavior and hippocampal extracellular ACh levels in male and female rats

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Publisher
Elsevier
Copyright
Copyright © 1999 Elsevier Ltd
ISSN
0006-8993
DOI
10.1016/S0006-8993(98)01171-8
Publisher site
See Article on Publisher Site

Abstract

In vivo microdialysis was used to assess the effects of novelty and pain on hippocampal ACh release in male and female rats. Experiments were carried out during the dark phase and consisted of 2 days of tests: on Day 1, after Baseline 1, animals were exposed to a new cage (Novelty) to which, 30 min later, a plastic cylinder (Object) was introduced. On Day 2, after Baseline 2, the Formalin test (50 μl of formalin 10%, s.c. injected in the dorsal hindpaw) was carried out in the animal's home cage. All behaviors were recorded. The extracellular levels of ACh in the dorsal hippocampus were estimated, in 10-min samples, by assay of ACh in the dialysates by HPLC. On Day 1 the raw values of ACh were higher in females than in males, but no sex difference was present when the percentage of change was considered. In both sexes the Novelty and Object tests induced an increase in ACh levels with respect to Baseline. Higher levels of exploration were present in females than males during the first 10 min of Novelty. On Day 2, ACh release increased in both sexes during the Formalin test. No sex difference in either ACh raw values or the percentages of change were found. Females showed higher levels of licking and lower levels of activity than males. The present study shows that novelty and pain induce similar hippocampal cholinergic activation in male and female rats but different behaviors. The results are discussed in light of the several anatomical and functional sex differences present in the hippocampus.

Journal

Brain ResearchElsevier

Published: Jan 9, 1999

References

  • Effects of novelty, pain and stress on hippocampal extracellular acetylcholine levels in male rats
    Aloisi, A.M.; Casamenti, F.; Scali, C.; Pepeu, G.; Carli, G.
  • Sex differences in pain-induced effects on the septo-hippocampal system
    Aloisi, A.M.
  • Sex differences in pain
    Berkley, K.J.
  • Effects of estrogen on potassium-stimulated acetylcholine release in the hippocampus and overlying cortex of adult rats
    Gibbs, R.B.; Hashash, A.; Johnson, D.A.
  • Estrogen facilitates induction of long term potentiation in the hippocampus of awake rats
    Montoya, D.A.C.; Carrer, H.F.

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