Effects of goat milk fractions on the stability of IGF-I in simulated gastrointestinal conditions

Effects of goat milk fractions on the stability of IGF-I in simulated gastrointestinal conditions The effects of goat milk fractions on the stability of insulin-like growth factor I (IGF-I) digested in the simulated gastrointestinal juice in vitro were investigated. IGF-I concentration was analyzed using an immunological sandwich-type ELISA method. The results showed that the concentration of pure IGF-I in simulated gastrointestinal juice decreased rapidly with the increasing digestion time. However, IGF-I in goat milk was more stable than pure IGF-I either in simulated gastric or intestinal juice. Goat milk and whey and whey supernatant fractions all effectively inhibited the proteolytic degradation of IGF-I in both the simulated gastric and intestinal juices. Dietary derived protease inhibitors, such as soya bean trypsin inhibitor (SBTI), lima bean trypsin inhibitor (LBTI) and egg white protease inhibitor (EWPI) all prevented IGF-I degradation in the simulated gastrointestinal juice, but the whey supernatant fraction represented more effective inhibition than the inhibitors. These results suggested that the whey supernatant fraction might contain specific inhibitors that protect IGF-I from the proteolytic degradation in the simulated gastrointestinal conditions, which was further verified by SDS-PAGE. Therefore, the whey supernatant fraction in goat milk could be a potential resource for the development of the effective oral IGF-I-based products. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png LWT - Food Science and Technology Elsevier

Effects of goat milk fractions on the stability of IGF-I in simulated gastrointestinal conditions

Loading next page...
 
/lp/elsevier/effects-of-goat-milk-fractions-on-the-stability-of-igf-i-in-simulated-aKhVfYDIza
Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier Ltd
ISSN
0023-6438
D.O.I.
10.1016/j.lwt.2018.01.017
Publisher site
See Article on Publisher Site

Abstract

The effects of goat milk fractions on the stability of insulin-like growth factor I (IGF-I) digested in the simulated gastrointestinal juice in vitro were investigated. IGF-I concentration was analyzed using an immunological sandwich-type ELISA method. The results showed that the concentration of pure IGF-I in simulated gastrointestinal juice decreased rapidly with the increasing digestion time. However, IGF-I in goat milk was more stable than pure IGF-I either in simulated gastric or intestinal juice. Goat milk and whey and whey supernatant fractions all effectively inhibited the proteolytic degradation of IGF-I in both the simulated gastric and intestinal juices. Dietary derived protease inhibitors, such as soya bean trypsin inhibitor (SBTI), lima bean trypsin inhibitor (LBTI) and egg white protease inhibitor (EWPI) all prevented IGF-I degradation in the simulated gastrointestinal juice, but the whey supernatant fraction represented more effective inhibition than the inhibitors. These results suggested that the whey supernatant fraction might contain specific inhibitors that protect IGF-I from the proteolytic degradation in the simulated gastrointestinal conditions, which was further verified by SDS-PAGE. Therefore, the whey supernatant fraction in goat milk could be a potential resource for the development of the effective oral IGF-I-based products.

Journal

LWT - Food Science and TechnologyElsevier

Published: May 1, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off