Effects of different mitogens on intrasplenic liver tissue transplants in comparison to orthotopic liver

Effects of different mitogens on intrasplenic liver tissue transplants in comparison to... Ectopic liver cell transplants, when compared to orthotopic liver, can serve as a tool to study topic influences on liver cell differentiation, multiplication, function and responsiveness to xenobiotics. The aim of the present study was to evaluate, if characteristic effects of mitogens are exerted in both liver and intrasplenic liver cell transplants in a similar manner. Fetal liver tissue suspensions were transplanted into the spleens of adult male syngenic rats. Four months later, transplant recipients and controls were treated with fluorene (FEN), fluorenone (FON), 2-acetylaminofluorene (AAF), N-nitrosodibenzylamine (NDBA) or the solvent 48 hours before sacrifice. The following parameters were assessed within livers and spleens: mitotic activity of hepatocytes, glycogen content, cytochrome P450 (P450) isoforms expression, P450 mediated monooxygenase functions, tissue content of lipid peroxides (LPO) and of reduced and oxidized glutathione (GSH; GSSG). In both orthotopic livers and intrasplenic transplants FEN, FON or NDBA administration increased the mitotic activity of the hepatocytes. Treatment with the mitogens caused a distinct and characteristic induction of the P450 isoforms expression and of the respective monooxygenase functions in the livers and (with certain differences) also in the transplants. FEN and FON slightly increased, AAF and NDBA reduced liver glycogen content. The latter effect was also seen in the transplants. NDBA administration caused a slight increase in tissue LPO content in livers, but not in spleens. Additionally, AAF or NDBA treatment led to an elevation of liver (but not of spleen) GSH and GSSG concentrations. The results of the present investigation show that characteristic effects of mitogens on orthotopic liver occur with certain differences also in ectopic liver cell transplants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Experimental and Toxicologic Pathology Elsevier

Effects of different mitogens on intrasplenic liver tissue transplants in comparison to orthotopic liver

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Publisher
Elsevier
Copyright
Copyright © 2003 Urban & Fischer Verlag
ISSN
0940-2993
eISSN
1618-1433
D.O.I.
10.1078/0940-2993-00282
Publisher site
See Article on Publisher Site

Abstract

Ectopic liver cell transplants, when compared to orthotopic liver, can serve as a tool to study topic influences on liver cell differentiation, multiplication, function and responsiveness to xenobiotics. The aim of the present study was to evaluate, if characteristic effects of mitogens are exerted in both liver and intrasplenic liver cell transplants in a similar manner. Fetal liver tissue suspensions were transplanted into the spleens of adult male syngenic rats. Four months later, transplant recipients and controls were treated with fluorene (FEN), fluorenone (FON), 2-acetylaminofluorene (AAF), N-nitrosodibenzylamine (NDBA) or the solvent 48 hours before sacrifice. The following parameters were assessed within livers and spleens: mitotic activity of hepatocytes, glycogen content, cytochrome P450 (P450) isoforms expression, P450 mediated monooxygenase functions, tissue content of lipid peroxides (LPO) and of reduced and oxidized glutathione (GSH; GSSG). In both orthotopic livers and intrasplenic transplants FEN, FON or NDBA administration increased the mitotic activity of the hepatocytes. Treatment with the mitogens caused a distinct and characteristic induction of the P450 isoforms expression and of the respective monooxygenase functions in the livers and (with certain differences) also in the transplants. FEN and FON slightly increased, AAF and NDBA reduced liver glycogen content. The latter effect was also seen in the transplants. NDBA administration caused a slight increase in tissue LPO content in livers, but not in spleens. Additionally, AAF or NDBA treatment led to an elevation of liver (but not of spleen) GSH and GSSG concentrations. The results of the present investigation show that characteristic effects of mitogens on orthotopic liver occur with certain differences also in ectopic liver cell transplants.

Journal

Experimental and Toxicologic PathologyElsevier

Published: Jan 1, 2003

References

  • Hepatocyte transplantation: an experimental study to treat acute liver failure in pigs
    Ambrosino, G.; Varotto, S.; Basso, S.
  • Intrasplenic transplantation of allogeneic hepatocytes prolongs survival in anhepatic rats
    Arkadopoulos, N.; Lilja, H.; Suh, K.S.
  • Hepatocyte transplantation in acute liver failure
    Bilir, B.M.; Guinette, D.; Karrer, F.
  • Induction of Cyp1a -1 and Cyp1a -2 gene expression by a reconstituted mixture of polynuclear aromatic hydrocarbons in B6C3F1 mice
    Chaloupka, K.; Steinberg, M.; Santostefano, M.
  • Mitogenic short-term effects on hepatocytes and adrenocortical cells: phenobarbital and reserpine compared to carcinogenic and non-carcinogenic fluorene derivatives
    Danz, M.; Hartmann, A.; Blaszyk, H.
  • Early initiating and promoting effects in 2-AAF-induced rat liver carcinogenesis: an immunohistochemical study
    Hadjiolov, N.; Bitsch, A.; Neumann, H.G.
  • Effect of hepatocyte growth factor on the proliferation of transplanted hepatocytes in the spleen
    Kato, K.; Kasai, S.; Onodera, K.
  • Long-term effects of intrasplenically transplanted adult hepatocytes and fetal liver in hyperbilirubinemic Gunn rats
    Kokudo, N.; Otsu, I.; Okazaki, T.
  • Developmental expression of cytochrome P450 isoforms after transplantation of fetal liver tissue suspension into the spleens of adult syngenic rats
    Lupp, A.; Lucas, N.; Lindström-Seppä, P.

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