Bioresource Technology 214 (2016) 411–418 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech Effective asymmetric bioreduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate by recombinant E. coli CCZU-A13 in [Bmim]PF -hydrolyzate media a,b,c,⇑ a a a a a Yu-Cai He , Zhi-Cheng Tao , Jun-Hua Di , Liang Chen , Lin-Bing Zhang , Dan-Ping Zhang , a a a a a,c Gang-Gang Chong , Feng Liu , Yun Ding , Chun-Xia Jiang , Cui-Luan Ma Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China Advanced Catalysis and Green Manufacturing Collaborative Innovation Center, Changzhou University, Changzhou, China Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, USA highl i ghts graphical a bstrac t Hydrolyzates promoted initial reaction rate and intracellular NADH content. Emulsiﬁer OP-10 (20 mM) increased the initial reaction rate of whole cells. Hp-b-cyclodextrin (0.010 mol Hp-b- CD/mol COBE) increased the initial reaction rate. Effective biosynthesis of (R)-CHBE (800 mM) was in [Bmim]PF - hydrolyzate media. article i nfo abstract Article history: It was the ﬁrst report that the concentrated hydrolyzates from the enzymatic hydrolysis of dilute NaOH Received 28 March 2016 (3 wt%)-soaking
Bioresource Technology – Elsevier
Published: Aug 1, 2016
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