ELSEVIER Effect of Hepatocyte Growth Factor on the Proliferation Transplanted in the Spleen K. Kato, S. Kasai, K. Onodera, M. Sawa, M. Imai, T. Kawahara, and M. Mito of EPATOCYTE growth factor (HGF), a multifunctional cytokine with mitogenic, monogenic, and morphogenic actions, represents the most potent stimulation of hepatocyte growth and DNA synthesis identified. It is becoming recognized as one of the most important factors in the regulation of liver regeneration after surgical resection or clinical damage. Experimental hepatocyte transplantation (HTx) is receiving increasing attention as a possible treatment for acute hepatic failure or enzyme-deficient intrasplenic HTx is limited by the diseases.â ,â However, relatively small number of cells3z4 hence rapid growth of transplanted into the spleen is required.5 In this study, we investigated the proliferative effect of continuous intravenous (IV) administration of recombinant human HGF (hHGF) on intrasplenically transplanted in rats. BrdU (50 mgikg) was administered IV to rats. Harvested spleens were sectioned, fixed in 70% ethanol, and stained via an immunohistochemical techniques using anti-BrdU monoclonal antibodies, avidin-biotinylated anti-mouse IgG, and a Vectastain ABC kit (Vector Lab, Burlingame, Calif). The number of labeled nuclei of was counted under light microscopy, and the labeling index (LI) was expressed
Transplantation Proceedings – Elsevier
Published: Jun 1, 1997
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