Effect of chronic paroxetine treatment on 5-HT 1B and 5-HT 1D autoreceptors in rat dorsal raphe nucleus

Effect of chronic paroxetine treatment on 5-HT 1B and 5-HT 1D autoreceptors in rat dorsal raphe... This study reports the effect of chronic paroxetine (10 mg/kg p.o., 21 days) on 5-HT 1B and 5-HT 1D autoreceptors controlling stimulated 5-HT efflux in slices of rat dorsal raphe nucleus. Electrically evoked 5-HT (10 pulses, 200 Hz, 0.1 ms, 10 mA) was measured using fast cyclic voltammetry. 5-HT efflux was inhibited by CP 93129 (10 nM–10 μM) and by sumatriptan (1 nM–1 μM) agonists at 5-HT 1B and 5-HT 1D receptors, respectively. Chronic paroxetine did not, initially, appear to alter the sensitivity of the 5-HT 1B autoreceptors to CP 93129. However, when constructed in the presence of WAY 100635 (10 nM) the selective and silent 5-HT 1A antagonist, there was a significant ( P <0.001) rightward shift of the CP 93129 concentration-response curve in the paroxetine-treated rats but not in the controls, implying a desensitisation of the 5-HT 1B autoreceptor by paroxetine. Chronic paroxetine did not affect the sumatriptan concentration-response curve, even with WAY 100635 present, implying that there was no (de)sensitisation of the 5-HT 1D autoreceptor. These data suggest that chronic paroxetine treatment may desensitise 5-HT 1B autoreceptors in the dorsal raphe nucleus but that this effect is unmasked only when the dominant 5-HT 1A autoreceptor control is antagonised. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neurochemistry International Elsevier

Effect of chronic paroxetine treatment on 5-HT 1B and 5-HT 1D autoreceptors in rat dorsal raphe nucleus

Loading next page...
 
/lp/elsevier/effect-of-chronic-paroxetine-treatment-on-5-ht-1b-and-5-ht-1d-nSLaeASUqn
Publisher
Elsevier
Copyright
Copyright © 2000 Elsevier Science Ltd
ISSN
0197-0186
DOI
10.1016/S0197-0186(99)00115-1
Publisher site
See Article on Publisher Site

Abstract

This study reports the effect of chronic paroxetine (10 mg/kg p.o., 21 days) on 5-HT 1B and 5-HT 1D autoreceptors controlling stimulated 5-HT efflux in slices of rat dorsal raphe nucleus. Electrically evoked 5-HT (10 pulses, 200 Hz, 0.1 ms, 10 mA) was measured using fast cyclic voltammetry. 5-HT efflux was inhibited by CP 93129 (10 nM–10 μM) and by sumatriptan (1 nM–1 μM) agonists at 5-HT 1B and 5-HT 1D receptors, respectively. Chronic paroxetine did not, initially, appear to alter the sensitivity of the 5-HT 1B autoreceptors to CP 93129. However, when constructed in the presence of WAY 100635 (10 nM) the selective and silent 5-HT 1A antagonist, there was a significant ( P <0.001) rightward shift of the CP 93129 concentration-response curve in the paroxetine-treated rats but not in the controls, implying a desensitisation of the 5-HT 1B autoreceptor by paroxetine. Chronic paroxetine did not affect the sumatriptan concentration-response curve, even with WAY 100635 present, implying that there was no (de)sensitisation of the 5-HT 1D autoreceptor. These data suggest that chronic paroxetine treatment may desensitise 5-HT 1B autoreceptors in the dorsal raphe nucleus but that this effect is unmasked only when the dominant 5-HT 1A autoreceptor control is antagonised.

Journal

Neurochemistry InternationalElsevier

Published: Feb 1, 2000

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off