Dual functioning of plant arginases provides a third route for putrescine synthesis

Dual functioning of plant arginases provides a third route for putrescine synthesis •ADC2 and ARGAH2 are localized to the chloroplast ADC2 and ARGAH genes enable putrescine synthesis in yeast mutants HPLC analysis shows that mixtures of ADC and ARGAH2 synthesize putrescine from arginine.•Agmatinase (ARGAH2) has a higher affinity for agmatine than Agmatine iminohydrolase (AIH) All ARGAH enzymes have arginase and agmatinase activity.•Michaelis–Menten parameters were calculated using a non-linear regression method and expressed as mean ± SE with GraphPad Prism software (http://www.graphpad.com/prism/Prism.htm). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Science Elsevier

Dual functioning of plant arginases provides a third route for putrescine synthesis

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Ltd
ISSN
0168-9452
D.O.I.
10.1016/j.plantsci.2017.05.011
Publisher site
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Abstract

•ADC2 and ARGAH2 are localized to the chloroplast ADC2 and ARGAH genes enable putrescine synthesis in yeast mutants HPLC analysis shows that mixtures of ADC and ARGAH2 synthesize putrescine from arginine.•Agmatinase (ARGAH2) has a higher affinity for agmatine than Agmatine iminohydrolase (AIH) All ARGAH enzymes have arginase and agmatinase activity.•Michaelis–Menten parameters were calculated using a non-linear regression method and expressed as mean ± SE with GraphPad Prism software (http://www.graphpad.com/prism/Prism.htm).

Journal

Plant ScienceElsevier

Published: Sep 1, 2017

References

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