Drosophila sickle Is a Novel grim-reaper Cell Death Activator

Drosophila sickle Is a Novel grim-reaper Cell Death Activator The Drosophila genes reaper , head involution defectiv e ( hid ), and grim all reside at 75C on chromosome three and encode related proteins that have crucial functions in programmed cell death (reviewed in (1–5) ). In this report, we describe a novel grim-reaper gene, termed sickle , that resides adjacent to reaper . The sickle gene, like reaper and grim , encodes a small protein which contains an RHG motif and a Trp-block. In wild-type embryos, sickle expression was detected in cells of the developing central nervous system. Unlike reaper , hid , and grim , the sickle gene is not removed by Df(3L)H99, and strong ectopic sickle expression was detected in the nervous system of this cell death mutant. sickle very effectively induced cell death in cultured Spodoptera Sf-9 cells, and this death was antagonized by the caspase inhibitors p35 or DIAP1. Strikingly, unlike the other grim-reaper genes, targeted sickle expression did not induce cell death in the Drosophila eye. However, sickle strongly enhanced the eye cell death induced by expression of either an r/grim chimera or reaper . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Biology Elsevier

Drosophila sickle Is a Novel grim-reaper Cell Death Activator

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Publisher
Elsevier
Copyright
Copyright © 2002 Cell Press
ISSN
0960-9822
DOI
10.1016/S0960-9822(01)00664-9
Publisher site
See Article on Publisher Site

Abstract

The Drosophila genes reaper , head involution defectiv e ( hid ), and grim all reside at 75C on chromosome three and encode related proteins that have crucial functions in programmed cell death (reviewed in (1–5) ). In this report, we describe a novel grim-reaper gene, termed sickle , that resides adjacent to reaper . The sickle gene, like reaper and grim , encodes a small protein which contains an RHG motif and a Trp-block. In wild-type embryos, sickle expression was detected in cells of the developing central nervous system. Unlike reaper , hid , and grim , the sickle gene is not removed by Df(3L)H99, and strong ectopic sickle expression was detected in the nervous system of this cell death mutant. sickle very effectively induced cell death in cultured Spodoptera Sf-9 cells, and this death was antagonized by the caspase inhibitors p35 or DIAP1. Strikingly, unlike the other grim-reaper genes, targeted sickle expression did not induce cell death in the Drosophila eye. However, sickle strongly enhanced the eye cell death induced by expression of either an r/grim chimera or reaper .

Journal

Current BiologyElsevier

Published: Jan 22, 2002

References

  • Regulation and execution of apoptosis during Drosophila development
    Bangs, P.; White, K.
  • Cell death regulation in Drosophila
    Vernooy, S.Y.; Copeland, J.; Ghaboosi, N.; Griffin, E.E.; Yoo, S.J.; Hay, B.A.
  • Induction of apoptosis by Drosophila reaper , hid and grim through inhibition of IAP function
    Goyal, L.; McCall, K.; Agapite, J.; Hartwieg, E.; Steller, H.
  • Diverse domains of THREAD/DIAP1 are required to inhibit apoptosis induced by REAPER and HID in Drosophila
    Lisi, S.; Mazzon, I.; White, K.

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