Double-barrel local perfusion system used to independently label the apical and the basal poles of outer hair cells isolated from the guinea-pig cochlea. Simultaneous perfusion from both barrels established laminar flow in front of the perfusor, ensuring that the opposite poles of this bipolar cell are labelled independently. The technique allows real time fluorescence imaging either 1) to investigate the apicobasal or the basoapical vesicle traffic along the entire length of the cell, or 2) to visualize simultaneously both apicobasal and basoapical vesicle traffic in the same cell.
Journal of Neuroscience Methods – Elsevier
Published: Jan 1, 2018
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