Double fluorescent labelling of a bipolar epithelial cell in vitro: The outer hair cell

Double fluorescent labelling of a bipolar epithelial cell in vitro: The outer hair cell Double-barrel local perfusion system used to independently label the apical and the basal poles of outer hair cells isolated from the guinea-pig cochlea. Simultaneous perfusion from both barrels established laminar flow in front of the perfusor, ensuring that the opposite poles of this bipolar cell are labelled independently. The technique allows real time fluorescence imaging either 1) to investigate the apicobasal or the basoapical vesicle traffic along the entire length of the cell, or 2) to visualize simultaneously both apicobasal and basoapical vesicle traffic in the same cell. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Methods Elsevier

Double fluorescent labelling of a bipolar epithelial cell in vitro: The outer hair cell

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Publisher
Elsevier
Copyright
Copyright © 2017 The Authors
ISSN
0165-0270
eISSN
1872-678X
D.O.I.
10.1016/j.jneumeth.2017.10.014
Publisher site
See Article on Publisher Site

Abstract

Double-barrel local perfusion system used to independently label the apical and the basal poles of outer hair cells isolated from the guinea-pig cochlea. Simultaneous perfusion from both barrels established laminar flow in front of the perfusor, ensuring that the opposite poles of this bipolar cell are labelled independently. The technique allows real time fluorescence imaging either 1) to investigate the apicobasal or the basoapical vesicle traffic along the entire length of the cell, or 2) to visualize simultaneously both apicobasal and basoapical vesicle traffic in the same cell.

Journal

Journal of Neuroscience MethodsElsevier

Published: Jan 1, 2018

References

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