DNA-triangular silver nanoparticles nanoprobe for the detection of dengue virus distinguishing serotype

DNA-triangular silver nanoparticles nanoprobe for the detection of dengue virus distinguishing... There is always a substantial effort to develop a point of care detection for a severe and acute disease like dengue. In this work, we have described the detection of serotype-specific dengue virus using multicolor triangular silver nanoparticles (TAg) which could be a potential diagnosis method to distinguish between serotype. Functionalized TAg was prepared with polyA10 DNA through pH-mediated process. Further, it was characterized with Transmission Electron Microscopy (TEM), Ultra Visible-visible spectroscopy (UV–vis) and gel electrophoresis and its stability towards NaCl concentration. The colorimetric detection was carried out based on the interaction of TAg-DNA probe with specific complimentary strand which was designed to form a network assembly between DNA probes and sample RNA. The hybridization process was enhanced in presence of NaCl (0.4 M) which makes the hybridization process more stable over probe compared to un-hybridization. Therefore a simple and effective colorimetric method was developed to detect the different dengue serotype RNA with high sensitivity and specificity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Elsevier

DNA-triangular silver nanoparticles nanoprobe for the detection of dengue virus distinguishing serotype

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier B.V.
ISSN
1386-1425
D.O.I.
10.1016/j.saa.2018.05.047
Publisher site
See Article on Publisher Site

Abstract

There is always a substantial effort to develop a point of care detection for a severe and acute disease like dengue. In this work, we have described the detection of serotype-specific dengue virus using multicolor triangular silver nanoparticles (TAg) which could be a potential diagnosis method to distinguish between serotype. Functionalized TAg was prepared with polyA10 DNA through pH-mediated process. Further, it was characterized with Transmission Electron Microscopy (TEM), Ultra Visible-visible spectroscopy (UV–vis) and gel electrophoresis and its stability towards NaCl concentration. The colorimetric detection was carried out based on the interaction of TAg-DNA probe with specific complimentary strand which was designed to form a network assembly between DNA probes and sample RNA. The hybridization process was enhanced in presence of NaCl (0.4 M) which makes the hybridization process more stable over probe compared to un-hybridization. Therefore a simple and effective colorimetric method was developed to detect the different dengue serotype RNA with high sensitivity and specificity.

Journal

Spectrochimica Acta Part A: Molecular and Biomolecular SpectroscopyElsevier

Published: Sep 5, 2018

References

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