DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo

DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway... •A phagemid-based deoxyinosine substrate can be repaired by minimal component reconstitute assay with endo V, polI and ligase.•The phagemid-based deoxyinosine substrate can be used for in vivo assay.•In vivo substrate with an additional C-C marker can identify strand loss associated with incomplete repair.•Using single nucleotide extension and MALDI-TOF MS mapped the 2-nucleotide excision track by pol I dI repair excision.•In vivo and in vitro results supported a complete mechanism for endo V repair pathway. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png DNA Repair Elsevier

DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier B.V.
ISSN
1568-7864
D.O.I.
10.1016/j.dnarep.2018.02.005
Publisher site
See Article on Publisher Site

Abstract

•A phagemid-based deoxyinosine substrate can be repaired by minimal component reconstitute assay with endo V, polI and ligase.•The phagemid-based deoxyinosine substrate can be used for in vivo assay.•In vivo substrate with an additional C-C marker can identify strand loss associated with incomplete repair.•Using single nucleotide extension and MALDI-TOF MS mapped the 2-nucleotide excision track by pol I dI repair excision.•In vivo and in vitro results supported a complete mechanism for endo V repair pathway.

Journal

DNA RepairElsevier

Published: Apr 1, 2018

References

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