•A phagemid-based deoxyinosine substrate can be repaired by minimal component reconstitute assay with endo V, polI and ligase.•The phagemid-based deoxyinosine substrate can be used for in vivo assay.•In vivo substrate with an additional C-C marker can identify strand loss associated with incomplete repair.•Using single nucleotide extension and MALDI-TOF MS mapped the 2-nucleotide excision track by pol I dI repair excision.•In vivo and in vitro results supported a complete mechanism for endo V repair pathway.
DNA Repair – Elsevier
Published: Apr 1, 2018
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