Distribution of aerosols in mouse lobes by fluorescent imaging

Distribution of aerosols in mouse lobes by fluorescent imaging Article history: Better methods are needed to quantify the distribution of drug among the airways of the lungs of small Received 25 October 2011 animals to facilitate the development of agents that can target specific airways. Mice were exposed to Received in revised form 13 January 2012 aerosols of aluminum phthalocyanine tetrasulfonic acid (AlPCS) that ranged in concentration and size Accepted 15 January 2012 (0.2–2.8 m). The trachea and lobes were removed and placed between glass slides, and fluorescent Available online 25 January 2012 images were obtained at two different compression thicknesses. The intensity, normalized by the area, exposure time, and thickness, was then plotted as a function of compression thickness, from which the Keywords: concentration and attenuation coefficient were estimated for each lobe and then for each pixel of the Fluorescent imaging image. The latter was then used to generate an image reflective of the concentration. The lobe volume, Lungs concentration, and tissue attenuation of AlPCS was consistent among the lobes. The deposition fraction Aerosol increased with decreasing particle size. The network of lines in the concentration image indicated that Aluminum phthalocyanine tetrasulfonic acid connective tissue has a lower concentration. The central airways were clearly evident in the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Pharmaceutics Elsevier

Distribution of aerosols in mouse lobes by fluorescent imaging

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Publisher
Elsevier
Copyright
Copyright © 2012 Elsevier B.V.
ISSN
0378-5173
D.O.I.
10.1016/j.ijpharm.2012.01.037
Publisher site
See Article on Publisher Site

Abstract

Article history: Better methods are needed to quantify the distribution of drug among the airways of the lungs of small Received 25 October 2011 animals to facilitate the development of agents that can target specific airways. Mice were exposed to Received in revised form 13 January 2012 aerosols of aluminum phthalocyanine tetrasulfonic acid (AlPCS) that ranged in concentration and size Accepted 15 January 2012 (0.2–2.8 m). The trachea and lobes were removed and placed between glass slides, and fluorescent Available online 25 January 2012 images were obtained at two different compression thicknesses. The intensity, normalized by the area, exposure time, and thickness, was then plotted as a function of compression thickness, from which the Keywords: concentration and attenuation coefficient were estimated for each lobe and then for each pixel of the Fluorescent imaging image. The latter was then used to generate an image reflective of the concentration. The lobe volume, Lungs concentration, and tissue attenuation of AlPCS was consistent among the lobes. The deposition fraction Aerosol increased with decreasing particle size. The network of lines in the concentration image indicated that Aluminum phthalocyanine tetrasulfonic acid connective tissue has a lower concentration. The central airways were clearly evident in the

Journal

International Journal of PharmaceuticsElsevier

Published: Apr 15, 2012

References

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