Discovery of a novel Picornavirales, Chequa iflavirus, from stressed redclaw crayfish (Cherax quadricarinatus) from farms in northern Queensland, Australia

Discovery of a novel Picornavirales, Chequa iflavirus, from stressed redclaw crayfish (Cherax... Virus Research 238 (2017) 148–155 Contents lists available at ScienceDirect Virus Research journal homepage: www.elsevier.com/locate/virusres Discovery of a novel Picornavirales, Chequa iflavirus, from stressed redclaw MARK crayfish (Cherax quadricarinatus) from farms in northern Queensland, Australia a,b a a, Kitikarn Sakuna , Jennifer Elliman , Leigh Owens College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, 4811, Australia Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, 80240, Thailand ARTICLE I NFO ABSTRACT Keywords: In 2014, northern Queensland crayfish from farms affected by particularly transportation and translocation Iflavirus stress, started to die with mortality reaching 20–40% after about three weeks and then mortalities subsided. Redclaw crayfish Crayfish from 1 farm had 65% mortalities within 11 weeks. With histological examination of broodstock and Cherax quadricarinatus juveniles, the muscle fibres were fractured with haemocytic infiltration reminiscent of viral infection or vitamin Stressed E/selenium deficiencies. Sequence dependent and independent PCRs failed to identify a viral aetiology. Chequa iflavirus However, the whole transcriptomes of a case crayfish and an unaffected crayfish from a different population Picornavirales were assembled producing over 500,000 contigs. The complete sequence of a positive sense, single stranded RNA virus (+ve ssRNA virus; 9933 bp) and the large http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Virus Research Elsevier

Discovery of a novel Picornavirales, Chequa iflavirus, from stressed redclaw crayfish (Cherax quadricarinatus) from farms in northern Queensland, Australia

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0168-1702
D.O.I.
10.1016/j.virusres.2017.06.021
Publisher site
See Article on Publisher Site

Abstract

Virus Research 238 (2017) 148–155 Contents lists available at ScienceDirect Virus Research journal homepage: www.elsevier.com/locate/virusres Discovery of a novel Picornavirales, Chequa iflavirus, from stressed redclaw MARK crayfish (Cherax quadricarinatus) from farms in northern Queensland, Australia a,b a a, Kitikarn Sakuna , Jennifer Elliman , Leigh Owens College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, 4811, Australia Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, 80240, Thailand ARTICLE I NFO ABSTRACT Keywords: In 2014, northern Queensland crayfish from farms affected by particularly transportation and translocation Iflavirus stress, started to die with mortality reaching 20–40% after about three weeks and then mortalities subsided. Redclaw crayfish Crayfish from 1 farm had 65% mortalities within 11 weeks. With histological examination of broodstock and Cherax quadricarinatus juveniles, the muscle fibres were fractured with haemocytic infiltration reminiscent of viral infection or vitamin Stressed E/selenium deficiencies. Sequence dependent and independent PCRs failed to identify a viral aetiology. Chequa iflavirus However, the whole transcriptomes of a case crayfish and an unaffected crayfish from a different population Picornavirales were assembled producing over 500,000 contigs. The complete sequence of a positive sense, single stranded RNA virus (+ve ssRNA virus; 9933 bp) and the large

Journal

Virus ResearchElsevier

Published: Jun 15, 2017

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