Differentiation of sturgeon species by PCR-RFLP

Differentiation of sturgeon species by PCR-RFLP A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNA Glu /cytochrome b ) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Food Research International Elsevier

Differentiation of sturgeon species by PCR-RFLP

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Publisher
Elsevier
Copyright
Copyright © 2000 Canadian Institute of Food Science and Technology
ISSN
0963-9969
D.O.I.
10.1016/S0963-9969(99)00150-7
Publisher site
See Article on Publisher Site

Abstract

A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNA Glu /cytochrome b ) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia.

Journal

Food Research InternationalElsevier

Published: Dec 1, 1999

References

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