Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length polymorphism

Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain... 16S rDNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to generate restriction profiles of the reference strains, including the American Type Culture Collection type strains, of oral Actinomyces spp., i.e., A . israelii, A. gerencseriae, A. naeslundii genospecies 1 and 2, A. odontolyticus, A. meyeri and A. georgiae , and 23 Actinomyces strains isolated from human dental plaque. The 16S rRNA gene sequences from isolated genomic DNA samples were amplified by PCR. The PCR products were purified and characterized by single digestion with four restriction endonucleases, i.e., Mnl I, Hae III, Cfo I, or Hpa II. Among them, Mnl I was found to discriminate the respective reference strains. The clinical isolates were assigned to one of the species, i.e., A. gerencseriae, A. naeslundii genospecies 1 and 2 and A. odontolyticus , on the basis of their restriction profiles by single digestion with Mnl I. Thus, 16S rDNA PCR-RFLP, using Mnl I, is a rapid and reliable method for the differentiation of oral Actinomyces spp. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Oral Biology Elsevier

Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length polymorphism

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Publisher
Elsevier
Copyright
Copyright © 1998 Elsevier Science Ltd.
ISSN
0003-9969
D.O.I.
10.1016/S0003-9969(98)00005-3
Publisher site
See Article on Publisher Site

Abstract

16S rDNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to generate restriction profiles of the reference strains, including the American Type Culture Collection type strains, of oral Actinomyces spp., i.e., A . israelii, A. gerencseriae, A. naeslundii genospecies 1 and 2, A. odontolyticus, A. meyeri and A. georgiae , and 23 Actinomyces strains isolated from human dental plaque. The 16S rRNA gene sequences from isolated genomic DNA samples were amplified by PCR. The PCR products were purified and characterized by single digestion with four restriction endonucleases, i.e., Mnl I, Hae III, Cfo I, or Hpa II. Among them, Mnl I was found to discriminate the respective reference strains. The clinical isolates were assigned to one of the species, i.e., A. gerencseriae, A. naeslundii genospecies 1 and 2 and A. odontolyticus , on the basis of their restriction profiles by single digestion with Mnl I. Thus, 16S rDNA PCR-RFLP, using Mnl I, is a rapid and reliable method for the differentiation of oral Actinomyces spp.

Journal

Archives of Oral BiologyElsevier

Published: Apr 1, 1998

References

  • An investigation into the use of restriction endonuclease analysis for the study of transmission of Actinomyces
    Barsotti, O.; Morrier, J.J.; Decoret, D.; Benay, G.; Rocca, J.P.
  • The impact of 16S ribosomal RNA-based phylogeny on the taxonomy of oral bacteria
    Tanner, A.; Maiden, M.F.J.; Paster, B.J.; Dewhirst, F.E.

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