Differential vulnerability of neurochemically identified subpopulations of retinal neurons in a monkey model of glaucoma

Differential vulnerability of neurochemically identified subpopulations of retinal neurons in a... The vulnerability of subpopulations of retinal neurons delineated by their content of cytoskeletal or calcium-binding proteins was evaluated in the retinas of cynomolgus monkeys in which glaucoma was produced with an argon laser. We quantitatively compared the number of neurons containing either neurofilament (NF) protein, parvalbumin, calbindin or calretinin immunoreactivity in central and peripheral portions of the nasal and temporal quadrants of the retina from glaucomatous and fellow non-glaucomatous eyes. There was no significant difference between the proportion of amacrine, horizontal and bipolar cells labeled with antibodies to the calcium-binding proteins comparing the two eyes. NF triplet immunoreactivity was present in a subpopulation of retinal ganglion cells, many of which, but not all, likely correspond to large ganglion cells that subserve the magnocellular visual pathway. Loss of NF protein-containing retinal ganglion cells was widespread throughout the central (59–77% loss) and peripheral (96–97%) nasal and temporal quadrants and was associated with the loss of NF-immunoreactive optic nerve fibers in the glaucomatous eyes. Comparison of counts of NF-immunoreactive neurons with total cell loss evaluated by Nissl staining indicated that NF protein-immunoreactive cells represent a large proportion of the cells that degenerate in the glaucomatous eyes, particularly in the peripheral regions of the retina. Such data may be useful in determining the cellular basis for sensitivity to this pathologic process and may also be helpful in the design of diagnostic tests that may be sensitive to the loss of the subset of NF-immunoreactive ganglion cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Brain Research Elsevier

Differential vulnerability of neurochemically identified subpopulations of retinal neurons in a monkey model of glaucoma

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Publisher
Elsevier
Copyright
Copyright © 1995 Elsevier Science B.V. All rights reserved
ISSN
0006-8993
D.O.I.
10.1016/0006-8993(95)00211-8
Publisher site
See Article on Publisher Site

Abstract

The vulnerability of subpopulations of retinal neurons delineated by their content of cytoskeletal or calcium-binding proteins was evaluated in the retinas of cynomolgus monkeys in which glaucoma was produced with an argon laser. We quantitatively compared the number of neurons containing either neurofilament (NF) protein, parvalbumin, calbindin or calretinin immunoreactivity in central and peripheral portions of the nasal and temporal quadrants of the retina from glaucomatous and fellow non-glaucomatous eyes. There was no significant difference between the proportion of amacrine, horizontal and bipolar cells labeled with antibodies to the calcium-binding proteins comparing the two eyes. NF triplet immunoreactivity was present in a subpopulation of retinal ganglion cells, many of which, but not all, likely correspond to large ganglion cells that subserve the magnocellular visual pathway. Loss of NF protein-containing retinal ganglion cells was widespread throughout the central (59–77% loss) and peripheral (96–97%) nasal and temporal quadrants and was associated with the loss of NF-immunoreactive optic nerve fibers in the glaucomatous eyes. Comparison of counts of NF-immunoreactive neurons with total cell loss evaluated by Nissl staining indicated that NF protein-immunoreactive cells represent a large proportion of the cells that degenerate in the glaucomatous eyes, particularly in the peripheral regions of the retina. Such data may be useful in determining the cellular basis for sensitivity to this pathologic process and may also be helpful in the design of diagnostic tests that may be sensitive to the loss of the subset of NF-immunoreactive ganglion cells.

Journal

Brain ResearchElsevier

Published: May 22, 1995

References

  • Binding of Bodian's silver and monoclonal antibodies to defined regions of human neurofilament subunits: Bodian's silver reacts with a highly charged unique domain of neurofilaments
    Autilio-Gambetti, L.; Crane, R.; Gambetti, P.
  • Immunocytochemical and biochemical localization of parvalbumin in the retina
    Endo, T.; Kobayashi, M.; Kobayashi, S.; Onaya, T.
  • Soma neurofilament immunoreactivity is related to cell size and fibre conduction velocity in rat primary sensory neurons
    Lawson, S.N.; Waddell, P.J.
  • How parallel are the primate visual pathways?
    Merigan, W.H.; Maunsell, J.H.R.
  • Neuronal transformations in Alzheimer's disease
    Metuzals, J.; Robitaille, Y.; Houghton, S.; Gauthier, S.; Kang, C.Y.; Leblanc, R.
  • The morphological correlates of X- and Y-like retinal ganglion cells in the retina of monkeys
    Perry, V.H.; Cowey, A.
  • Horizontal cells in the monkey retina: immunocytochemical staining with antibodies against calcium binding proteins
    Ro¨hrenbeck, J.; Wa¨ssle, H.; Boycott, B.B.
  • Visual sensitivity and parallel retinocortical channels
    Shapley, R.
  • Neurofilament protein triplet immunoreactivity in the dorsal root ganglia of the guinea-pig
    Vickers, J.C.; Costa, M.

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