Differential expression of cannabinoid CB 2 receptor mRNA in mouse immune cell subpopulations and following B cell stimulation

Differential expression of cannabinoid CB 2 receptor mRNA in mouse immune cell subpopulations and... Cannabinoid CB 2 receptor is reported to be expressed in varying amounts in different human immune subpopulations. To examine the expression pattern of CB 2 in the mouse, immune cell subpopulations were purified and studied by semiquantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR). CB 2 mRNA was most abundant in splenic B cells, followed by macrophages and T cells. Furthermore, CB 2 was expressed in thioglycollate-elicited peritoneal macrophages, but not in resident peritoneal macrophages. In addition to these studies on receptor expression at basal activity, CB 2 mRNA expression was also studied following immune cell activation. Bacterial lipopolysaccharide stimulation downregulated CB 2 mRNA expression in splenocyte cultures in a dose–response manner, while stimulation through cluster of differentiation 40 (CD40) using anti-CD40 antibody upregulated the response and costimulation with interleukin-4 attenuated the anti-CD40 response. These results demonstrate that CB 2 mRNA expression differs among mouse immune subpopulations similar to what is observed in human immune cells. Furthermore, the results suggest that the signaling pathways activated by lipopolysaccharide and anti-CD40 might have different effects on CB 2 mRNA expression. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Pharmacology Elsevier

Differential expression of cannabinoid CB 2 receptor mRNA in mouse immune cell subpopulations and following B cell stimulation

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Publisher
Elsevier
Copyright
Copyright © 2001 Elsevier Ltd
ISSN
0014-2999
D.O.I.
10.1016/S0014-2999(01)01122-0
Publisher site
See Article on Publisher Site

Abstract

Cannabinoid CB 2 receptor is reported to be expressed in varying amounts in different human immune subpopulations. To examine the expression pattern of CB 2 in the mouse, immune cell subpopulations were purified and studied by semiquantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR). CB 2 mRNA was most abundant in splenic B cells, followed by macrophages and T cells. Furthermore, CB 2 was expressed in thioglycollate-elicited peritoneal macrophages, but not in resident peritoneal macrophages. In addition to these studies on receptor expression at basal activity, CB 2 mRNA expression was also studied following immune cell activation. Bacterial lipopolysaccharide stimulation downregulated CB 2 mRNA expression in splenocyte cultures in a dose–response manner, while stimulation through cluster of differentiation 40 (CD40) using anti-CD40 antibody upregulated the response and costimulation with interleukin-4 attenuated the anti-CD40 response. These results demonstrate that CB 2 mRNA expression differs among mouse immune subpopulations similar to what is observed in human immune cells. Furthermore, the results suggest that the signaling pathways activated by lipopolysaccharide and anti-CD40 might have different effects on CB 2 mRNA expression.

Journal

European Journal of PharmacologyElsevier

Published: Jul 6, 2001

References

  • CD40-mediated stimulation of B1 and B2 cells: implication in autoantibody production in murine lupus
    Kaneko, Y.; Hirose, S.; Abe, M.; Yagita, H.; Okumura, K.; Shirai, T.
  • Recombinant CD40 ligand stimulation of murine B cell growth and differentiation: cooperative effects of cytokines
    Maliszewski, C.R.; Grabstein, K.; Fanslow, W.C.; Armitage, R.; Spriggs, M.K.; Sato, T.A.
  • Functions of CD40 on B cells, dendritic cells and other cells
    van Kooten, C.; Banchereau, J.
  • The activation antigen CD69
    Ziegler, S.F.; Ramsdell, F.; Alderson, M.R.

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