Differential DNA binding activities of the transcription factors AP-1 and Oct-1 during light-induced apoptosis of photoreceptors

Differential DNA binding activities of the transcription factors AP-1 and Oct-1 during... The activity of transcription factors like AP-1 and Oct-1 is critical for the regulation of gene expression. Whereas Oct-1 mainly regulates the expression of housekeeping genes, AP-1 is often involved in cellular responses to external stimuli and plays an essential role in the regulation of light-induced apoptosis of mouse retinal photoreceptors. In this study, we investigated AP-1 and Oct-1 DNA binding activity and AP-1 complex composition in the mouse retina during light-induced photoreceptor apoptosis. AP-1 DNA binding activity was low in dark-adapted animals but was transiently elevated within 12 h after exposure of mice to apoptosis-inducing levels of white fluorescent light. Maximal AP-1 activity was found 6 h after light-exposure. Antibody interference analysis at 6 h after damaging light exposure and under normal light conditions revealed that the major fraction of AP-1 consists of c-Fos/JunD heterodimers in both situations. In contrast to AP-1, Oct-1 DNA binding activity was maximal in dark-adapted animals and was reduced during photoreceptor apoptosis. Transient induction of AP-1 (c-Fos/JunD) and inactivation of Oct-1 may be crucial events for light-mediated apoptosis of retinal photoreceptors. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Vision Research Elsevier

Differential DNA binding activities of the transcription factors AP-1 and Oct-1 during light-induced apoptosis of photoreceptors

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Publisher
Elsevier
Copyright
Copyright © 1999 Elsevier Science Ltd
ISSN
0042-6989
eISSN
1878-5646
DOI
10.1016/S0042-6989(98)00313-7
Publisher site
See Article on Publisher Site

Abstract

The activity of transcription factors like AP-1 and Oct-1 is critical for the regulation of gene expression. Whereas Oct-1 mainly regulates the expression of housekeeping genes, AP-1 is often involved in cellular responses to external stimuli and plays an essential role in the regulation of light-induced apoptosis of mouse retinal photoreceptors. In this study, we investigated AP-1 and Oct-1 DNA binding activity and AP-1 complex composition in the mouse retina during light-induced photoreceptor apoptosis. AP-1 DNA binding activity was low in dark-adapted animals but was transiently elevated within 12 h after exposure of mice to apoptosis-inducing levels of white fluorescent light. Maximal AP-1 activity was found 6 h after light-exposure. Antibody interference analysis at 6 h after damaging light exposure and under normal light conditions revealed that the major fraction of AP-1 consists of c-Fos/JunD heterodimers in both situations. In contrast to AP-1, Oct-1 DNA binding activity was maximal in dark-adapted animals and was reduced during photoreceptor apoptosis. Transient induction of AP-1 (c-Fos/JunD) and inactivation of Oct-1 may be crucial events for light-mediated apoptosis of retinal photoreceptors.

Journal

Vision ResearchElsevier

Published: Jul 1, 1999

References

  • Induction of apoptosis by the transcription factor c-Jun
    Bossy Wetzel, E.; Bakiri, L.; Yaniv, M.
  • Temporal analysis of events associated with programmed cell death (apoptosis) of sympathetic neurons deprived of nerve growth factor
    Deckwerth, T.L.; Johnson, E.M.
  • Altered gene expression in neurons during programmed cell death: identification of c-jun as necessary for neuronal apoptosis
    Estus, S.; Zaks, W.J.; Freeman, R.S.; Gruda, M.; Bravo, R.; Johnson, E.M.
  • Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis
    Haimovitz Friedman, A.; Kan, C.C.; Ehleiter, D.; Persaud, R.S.; McLoughlin, M.; Fuks, Z.; Kolesnick, R.N.

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