Dietary xenoestrogens differentially impair 3T3-L1 preadipocyte differentiation and persistently affect leptin synthesis

Dietary xenoestrogens differentially impair 3T3-L1 preadipocyte differentiation and persistently... Recent observations have highlighted adipogenesis alterations under exposure to several xenoestrogens at critical stages, and pointed at their possible involvement in the pathogenesis of obesity. However, it remains unclear whether these effects are mediated by classical estrogen receptor (ER) binding and subsequent transcriptional modulation. The aim of this study was to determine the (anti-)adipogenic impact of apigenin, bisphenol A, genistein and 17β-estradiol at the onset of adipose cell maturation, and to correlate it to their estrogenic potential. In steroid-free conditions, 3T3-L1 preadipocytes were induced to differentiate in the presence of xenoestrogens for 2 days. DNA and triglyceride levels, leptin secretion and expression of Pref-1, C/EBPβ, PPARγ2, FAS, leptin and ERs were measured on days 0, 3 and 8 of differentiation. Genistein potently blocked mitotic clonal expansion and all markers of maturation. Bisphenol A and estradiol did not modify triglyceride accumulation but increased the expression of differentiation genes. Apigenin caused a weak but reversible delay in adipogenesis although it unexpectedly enhanced leptin synthesis. However, the expression of steroid hormone receptors was not associated with these differential effects. In conclusion, we could not put a clear estrogen-dependent mechanism forward, but early exposure to xenoestrogens persistently disrupted adipocyte gene expression and leptin synthesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Steroid Biochemistry and Molecular Biology Elsevier

Dietary xenoestrogens differentially impair 3T3-L1 preadipocyte differentiation and persistently affect leptin synthesis

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Publisher
Elsevier
Copyright
Copyright © 2008 Elsevier Ltd
ISSN
0960-0760
eISSN
1879-1220
DOI
10.1016/j.jsbmb.2008.02.006
Publisher site
See Article on Publisher Site

Abstract

Recent observations have highlighted adipogenesis alterations under exposure to several xenoestrogens at critical stages, and pointed at their possible involvement in the pathogenesis of obesity. However, it remains unclear whether these effects are mediated by classical estrogen receptor (ER) binding and subsequent transcriptional modulation. The aim of this study was to determine the (anti-)adipogenic impact of apigenin, bisphenol A, genistein and 17β-estradiol at the onset of adipose cell maturation, and to correlate it to their estrogenic potential. In steroid-free conditions, 3T3-L1 preadipocytes were induced to differentiate in the presence of xenoestrogens for 2 days. DNA and triglyceride levels, leptin secretion and expression of Pref-1, C/EBPβ, PPARγ2, FAS, leptin and ERs were measured on days 0, 3 and 8 of differentiation. Genistein potently blocked mitotic clonal expansion and all markers of maturation. Bisphenol A and estradiol did not modify triglyceride accumulation but increased the expression of differentiation genes. Apigenin caused a weak but reversible delay in adipogenesis although it unexpectedly enhanced leptin synthesis. However, the expression of steroid hormone receptors was not associated with these differential effects. In conclusion, we could not put a clear estrogen-dependent mechanism forward, but early exposure to xenoestrogens persistently disrupted adipocyte gene expression and leptin synthesis.

Journal

The Journal of Steroid Biochemistry and Molecular BiologyElsevier

Published: May 1, 2008

References

  • Endocrine disrupters: a human risk?
    Waring, R.H.; Harris, R.M.
  • Differential effects of flavonoids on 3T3-L1 adipogenesis and lipolysis
    Harmon, A.W.; Harp, J.B.
  • Depot differences in steroid receptor expression in adipose tissue: possible role of the local steroid milieu
    Rodriguez-Cuenca, S.; Monjo, M.; Proenza, A.M.; Roca, P.
  • Understanding adipocyte differentiation
    Gregoire, F.M.; Smas, C.M.; Sul, H.S.
  • Assessment of total effective xenoestrogen burden in adipose tissue and identification of chemicals responsible for the combined estrogenic effect
    Fernandez, M.F.; Rivas, A.; Olea-Serrano, F.; Cerrillo, I.; Molina-Molina, J.M.; Araque, P.; Martinez-Vidal, J.L.; Olea, N.
  • Regulation of leptin by steroid hormones in rat adipose tissue
    Kristensen, K.; Pedersen, S.B.; Richelsen, B.

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