Development of RT-qPCR assays for the specific identification of two major genotypes of avian infectious bronchitis virus

Development of RT-qPCR assays for the specific identification of two major genotypes of avian... •RT-qPCR assays were developed for the detection of two major IBV genotypes.•The assays use TaqMan probes that target sequences in the spike glycoprotein gene.•The RT-qPCR assays were specific, sensitive and reproducible.•A large number of clinical samples can be easily screened.•The assays are expected to improve our understanding of IBV epidemiology. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

Development of RT-qPCR assays for the specific identification of two major genotypes of avian infectious bronchitis virus

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Publisher
Elsevier
Copyright
Copyright © 2016 Elsevier B.V.
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2016.05.007
Publisher site
See Article on Publisher Site

Abstract

•RT-qPCR assays were developed for the detection of two major IBV genotypes.•The assays use TaqMan probes that target sequences in the spike glycoprotein gene.•The RT-qPCR assays were specific, sensitive and reproducible.•A large number of clinical samples can be easily screened.•The assays are expected to improve our understanding of IBV epidemiology.

Journal

Journal of Virological MethodsElsevier

Published: Sep 1, 2016

References

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