Development of imidazolinone herbicide tolerant borage (Borago officinalis L.)

Development of imidazolinone herbicide tolerant borage (Borago officinalis L.) •Borage is commercially cultivated for gamma-linolenic acid (GLA, 18:3-6,9,2), a nutraceutical fatty acid used in food and cosmetics.•However, an herbicide-resistant variety for effective weed management has not yet been developed, which severely constrains the borage framing.•This manuscript reports generation of two borage mutant lines resistant to herbicide imidazolinone by ethyl methanesulfonate (EMS) mutagenesis.•Molecular analysis of the mutant lines revealed single nucleotide substitution inside two acetohydroxyacid synthase (AHAS) genes.•A KASP marker was thus developed to differentiate the homozygous susceptible, homozygous resistant and heterozygous borage plants.•An in vitro assay further showed that homozygous resistant borage carrying the AHAS1 mutation retained significantly higher AHAS activity than susceptible borage across different imazamox concentrations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Science Elsevier

Development of imidazolinone herbicide tolerant borage (Borago officinalis L.)

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Ltd
ISSN
0168-9452
D.O.I.
10.1016/j.plantsci.2017.05.012
Publisher site
See Article on Publisher Site

Abstract

•Borage is commercially cultivated for gamma-linolenic acid (GLA, 18:3-6,9,2), a nutraceutical fatty acid used in food and cosmetics.•However, an herbicide-resistant variety for effective weed management has not yet been developed, which severely constrains the borage framing.•This manuscript reports generation of two borage mutant lines resistant to herbicide imidazolinone by ethyl methanesulfonate (EMS) mutagenesis.•Molecular analysis of the mutant lines revealed single nucleotide substitution inside two acetohydroxyacid synthase (AHAS) genes.•A KASP marker was thus developed to differentiate the homozygous susceptible, homozygous resistant and heterozygous borage plants.•An in vitro assay further showed that homozygous resistant borage carrying the AHAS1 mutation retained significantly higher AHAS activity than susceptible borage across different imazamox concentrations.

Journal

Plant ScienceElsevier

Published: Sep 1, 2017

References

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