Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated with severe diarrhea disease in pigs

Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated... •We established a TaqMan-based real-time RT-PCR assay for the detection of a novel swine diarrhea virus SADS-CoV.•The assay in this study was of high sensitivity, specificity and reproducibility.•We compared the accuracy between conventional PCR and TaqMan-based RT-PCR assay with adequate clinical samples.•This approach will help to provide knowledge on epidemiology and pathogenesis studies of SADS-CoV. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated with severe diarrhea disease in pigs

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier B.V.
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2018.02.002
Publisher site
See Article on Publisher Site

Abstract

•We established a TaqMan-based real-time RT-PCR assay for the detection of a novel swine diarrhea virus SADS-CoV.•The assay in this study was of high sensitivity, specificity and reproducibility.•We compared the accuracy between conventional PCR and TaqMan-based RT-PCR assay with adequate clinical samples.•This approach will help to provide knowledge on epidemiology and pathogenesis studies of SADS-CoV.

Journal

Journal of Virological MethodsElsevier

Published: May 1, 2018

References

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