•We established a TaqMan-based real-time RT-PCR assay for the detection of a novel swine diarrhea virus SADS-CoV.•The assay in this study was of high sensitivity, specificity and reproducibility.•We compared the accuracy between conventional PCR and TaqMan-based RT-PCR assay with adequate clinical samples.•This approach will help to provide knowledge on epidemiology and pathogenesis studies of SADS-CoV.
Journal of Virological Methods – Elsevier
Published: May 1, 2018
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