Virus Research 243 (2018) 69–74 Contents lists available at ScienceDirect Virus Research journal homepage: www.elsevier.com/locate/virusres Development of a rapid and quantitative method for the analysis of viral MARK entry and release using a NanoLuc luciferase complementation assay a a a b,c Michihito Sasaki , Paulina D. Anindita , Wallaya Phongphaew , Michael Carr , d a a,b,e, Shintaro Kobayashi , Yasuko Orba , Hirofumi Sawa Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo 001-0020, Japan National Virus Reference Laboratory, University College of Dublin, Dublin 4, Ireland Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan Global Virus Network, Baltimore, MD 21201, USA ARTICLE I NFO ABSTRACT Keywords: Subviral particles (SVPs) self-assemble and are released from cells transfected with expression plasmids encoding Flavivirus ﬂavivirus structural proteins. Flavivirus-like particles (VLPs), consisting of ﬂavivirus structural proteins and a Virus entry subgenomic replicon, can enter cells and cause single-round infections. Neither SVPs or VLPs possess complete Virus release viral RNA genomes, therefore are replication-incompetent systems; however, they retain the capacity to fuse and Luciferase complementation assay bud from target cells and follow
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