Development of a rapid and quantitative method for the analysis of viral entry and release using a NanoLuc luciferase complementation assay

Development of a rapid and quantitative method for the analysis of viral entry and release using... •SVPs and VLPs harboring the HiBiT tag (SVP-HiBiT and VLP-HiBiT) were generated.•The budded SVP-HiBiT in the supernatant could be readily quantified by luminometry.•The cellular entry of VLP-HiBiT was monitored in live-cell conditions by luminometry.•Proof-of-principle of the methods utilizing SVP-HiBiT and VLP-HiBiT was demonstrated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Virus Research Elsevier

Development of a rapid and quantitative method for the analysis of viral entry and release using a NanoLuc luciferase complementation assay

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0168-1702
D.O.I.
10.1016/j.virusres.2017.10.015
Publisher site
See Article on Publisher Site

Abstract

•SVPs and VLPs harboring the HiBiT tag (SVP-HiBiT and VLP-HiBiT) were generated.•The budded SVP-HiBiT in the supernatant could be readily quantified by luminometry.•The cellular entry of VLP-HiBiT was monitored in live-cell conditions by luminometry.•Proof-of-principle of the methods utilizing SVP-HiBiT and VLP-HiBiT was demonstrated.

Journal

Virus ResearchElsevier

Published: Jan 2, 2018

References

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