The relative density of lymphocyte CD3, CD4, CD5, CD8, CD20, CD23, CD28, CD38, CD45RA, CD45RO, CD57 and HLA-DR antigens was measured as antibody binding capacity (ABC) in 58 blood donors aged 19–66 years. The group was analysed in order to obtain reference values (percentages and absolute numbers) for routine, quantitative three-colour flow cytometry (FC) tests, and we included around ten males and females for each of the 15 year age intervals. Whole blood was stained (30 min on ice) with FITC, PE or PerCP conjugated MAbs. The analysis was performed with a FACScan equipped with LYSYS II and Paint-a-Gate Plus software. The instrument was calibrated daily with QC3, QuickCal (FITC and PE) and Calibrite and monthly with QSC and stained cells (which included also the control for PerCP performance). The ABC was measured with QSC (Flow Cytometry Standards Corporation). The CD4 + lymphocytes expressed significantly more CD3, CD28 and HLA-DR antigens, and less CD45RA antigen than the CD8 + cells ( p < 0.0001). A significant decrease with age was observed for CD3 and CD45RA on both CD4 + and CD8 + subsets ( p < 0.05). The lymphocytes of women, compared with those of men, showed decreased ABC for CD8, CD20 and CD28 antigens. The results illustrate the necessity for close matching of control with case groups. They also illustrate the possibilities of modern FC methods based on quantitative quality control and three-colour analysis.
Journal of Immunological Methods – Elsevier
Published: Jun 28, 1995
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera