Determination of the antibody binding capacity of lymphocyte membrane antigens by flow cytometry in 58 blood donors

Determination of the antibody binding capacity of lymphocyte membrane antigens by flow cytometry... The relative density of lymphocyte CD3, CD4, CD5, CD8, CD20, CD23, CD28, CD38, CD45RA, CD45RO, CD57 and HLA-DR antigens was measured as antibody binding capacity (ABC) in 58 blood donors aged 19–66 years. The group was analysed in order to obtain reference values (percentages and absolute numbers) for routine, quantitative three-colour flow cytometry (FC) tests, and we included around ten males and females for each of the 15 year age intervals. Whole blood was stained (30 min on ice) with FITC, PE or PerCP conjugated MAbs. The analysis was performed with a FACScan equipped with LYSYS II and Paint-a-Gate Plus software. The instrument was calibrated daily with QC3, QuickCal (FITC and PE) and Calibrite and monthly with QSC and stained cells (which included also the control for PerCP performance). The ABC was measured with QSC (Flow Cytometry Standards Corporation). The CD4 + lymphocytes expressed significantly more CD3, CD28 and HLA-DR antigens, and less CD45RA antigen than the CD8 + cells ( p < 0.0001). A significant decrease with age was observed for CD3 and CD45RA on both CD4 + and CD8 + subsets ( p < 0.05). The lymphocytes of women, compared with those of men, showed decreased ABC for CD8, CD20 and CD28 antigens. The results illustrate the necessity for close matching of control with case groups. They also illustrate the possibilities of modern FC methods based on quantitative quality control and three-colour analysis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Immunological Methods Elsevier

Determination of the antibody binding capacity of lymphocyte membrane antigens by flow cytometry in 58 blood donors

Loading next page...
 
/lp/elsevier/determination-of-the-antibody-binding-capacity-of-lymphocyte-membrane-4vJqoTuX94
Publisher
Elsevier
Copyright
Copyright © 1995 Elsevier Ltd
ISSN
0022-1759
DOI
10.1016/0022-1759(95)00064-H
Publisher site
See Article on Publisher Site

Abstract

The relative density of lymphocyte CD3, CD4, CD5, CD8, CD20, CD23, CD28, CD38, CD45RA, CD45RO, CD57 and HLA-DR antigens was measured as antibody binding capacity (ABC) in 58 blood donors aged 19–66 years. The group was analysed in order to obtain reference values (percentages and absolute numbers) for routine, quantitative three-colour flow cytometry (FC) tests, and we included around ten males and females for each of the 15 year age intervals. Whole blood was stained (30 min on ice) with FITC, PE or PerCP conjugated MAbs. The analysis was performed with a FACScan equipped with LYSYS II and Paint-a-Gate Plus software. The instrument was calibrated daily with QC3, QuickCal (FITC and PE) and Calibrite and monthly with QSC and stained cells (which included also the control for PerCP performance). The ABC was measured with QSC (Flow Cytometry Standards Corporation). The CD4 + lymphocytes expressed significantly more CD3, CD28 and HLA-DR antigens, and less CD45RA antigen than the CD8 + cells ( p < 0.0001). A significant decrease with age was observed for CD3 and CD45RA on both CD4 + and CD8 + subsets ( p < 0.05). The lymphocytes of women, compared with those of men, showed decreased ABC for CD8, CD20 and CD28 antigens. The results illustrate the necessity for close matching of control with case groups. They also illustrate the possibilities of modern FC methods based on quantitative quality control and three-colour analysis.

Journal

Journal of Immunological MethodsElsevier

Published: Jun 28, 1995

References

  • Postnatal expansion of the natural killer and killer cell population in humans identified by the monoclonal HNK-1 antibody
    Abo, T.; Cooper, M.D.; Balch, C.M.
  • A comprehensive immunological analysis in chronic fatigue syndrome
    Gupta, S.; Vayuvegula, B.
  • Lymphocyte activation and effector functions
    Janeway, C.A.; Golstein, P.
  • CD3-T cell receptor modulation is selectively induced in CD8 but not CD4 lymphocytes cultured in agar
    Oudrhiri, N.; Farcet, J.P.; Gourdin, M.F.; M'Bemba, E.; Gaulard, P.; Katz, A.; Divine, M.; Galazka, A.; Reyes, F.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off