Determination of conventional protein kinase C isoforms involved in high intraocular pressure-induced retinal ischemic preconditioning of rats

Determination of conventional protein kinase C isoforms involved in high intraocular... Evidence indicates that conventional protein kinase C (cPKC) plays a pivotal role in the development of retinal ischemic preconditioning (IPC). In this study, the effect of high intraocular pressure (IOP)-induced retinal IPC on cPKC isoform-specific membrane translocation and protein expression were observed. We found that cPKCγ membrane translocation increased significantly at the early stage (20 min-1 h), while the protein expression levels of cPKCα and γ were markedly elevated in the delayed retinal IPC (12–168 h) of rats. The increased protein expressions of cPKCα at 72 h and cPKCγ at 24 h after IPC were further confirmed by immunofluorescence staining. In addition, we found that cPKCγ co-localized with retinal ganglion cell (RGC)-specific marker, neurofilaments heavy chain (NF-H) by using double immunofluorescence labeling. These results suggest that increased cPKCγ membrane translocation and up-regulated protein expressions of cPKCα and γ are involved in the development of high IOP-induced rat retinal IPC. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Vision Research Elsevier

Determination of conventional protein kinase C isoforms involved in high intraocular pressure-induced retinal ischemic preconditioning of rats

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Publisher
Elsevier
Copyright
Copyright © 2008 Elsevier Ltd
ISSN
0042-6989
eISSN
1878-5646
D.O.I.
10.1016/j.visres.2008.10.018
Publisher site
See Article on Publisher Site

Abstract

Evidence indicates that conventional protein kinase C (cPKC) plays a pivotal role in the development of retinal ischemic preconditioning (IPC). In this study, the effect of high intraocular pressure (IOP)-induced retinal IPC on cPKC isoform-specific membrane translocation and protein expression were observed. We found that cPKCγ membrane translocation increased significantly at the early stage (20 min-1 h), while the protein expression levels of cPKCα and γ were markedly elevated in the delayed retinal IPC (12–168 h) of rats. The increased protein expressions of cPKCα at 72 h and cPKCγ at 24 h after IPC were further confirmed by immunofluorescence staining. In addition, we found that cPKCγ co-localized with retinal ganglion cell (RGC)-specific marker, neurofilaments heavy chain (NF-H) by using double immunofluorescence labeling. These results suggest that increased cPKCγ membrane translocation and up-regulated protein expressions of cPKCα and γ are involved in the development of high IOP-induced rat retinal IPC.

Journal

Vision ResearchElsevier

Published: Feb 1, 2009

References

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