Detection of Strawberry necrotic shock virus using conventional and TaqMan® quantitative RT-PCR

Detection of Strawberry necrotic shock virus using conventional and TaqMan® quantitative RT-PCR •New universal detection protocols have been developed for Strawberry necrotic shock virus.•Conventional PCR can detect as low as 4pg/μl of virus RNA; qPCR 40fg/μl of virus RNA.•The assays have been successfully used on field material.•This research has major implications for certification programs around the world. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

Detection of Strawberry necrotic shock virus using conventional and TaqMan® quantitative RT-PCR

Loading next page...
 
/lp/elsevier/detection-of-strawberry-necrotic-shock-virus-using-conventional-and-STOiBnvjyj
Publisher
Elsevier
Copyright
Copyright © 2016 Elsevier B.V.
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2016.06.005
Publisher site
See Article on Publisher Site

Abstract

•New universal detection protocols have been developed for Strawberry necrotic shock virus.•Conventional PCR can detect as low as 4pg/μl of virus RNA; qPCR 40fg/μl of virus RNA.•The assays have been successfully used on field material.•This research has major implications for certification programs around the world.

Journal

Journal of Virological MethodsElsevier

Published: Sep 1, 2016

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial