Degranulation and superoxide production depend on cholesterol in PLB-985 cells

Degranulation and superoxide production depend on cholesterol in PLB-985 cells The effect of agents disrupting cholesterol-rich microdomains of the cell membrane was studied on the chemoattractant receptor (FPR and FRPL1) coupled effector responses of promyelocytic PLB-985 cells. Both methyl-β-cyclodextrin (MβCD) and filipin III inhibited exocytosis of primary granules and O 2 − -production induced by stimulation of either chemotactic receptor. Alteration of calcium homeostasis of MβCD-treated cells does not account for the impairment of the effector responses. Disruption of microfilaments by cytochalasin B (CB) partially reverses the inhibitory effect of cholesterol depletion. Our results provide functional support for the involvement of cholesterol-rich membrane domains in the signaling of chemotactic receptors and call the attention to the possible role of microfilaments in the organization of lipid microdomains. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochemical and Biophysical Research Communications Elsevier

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Publisher
Elsevier
Copyright
Copyright © 2003 Elsevier Inc.
ISSN
0006-291x
D.O.I.
10.1016/j.bbrc.2003.09.150
Publisher site
See Article on Publisher Site

Abstract

The effect of agents disrupting cholesterol-rich microdomains of the cell membrane was studied on the chemoattractant receptor (FPR and FRPL1) coupled effector responses of promyelocytic PLB-985 cells. Both methyl-β-cyclodextrin (MβCD) and filipin III inhibited exocytosis of primary granules and O 2 − -production induced by stimulation of either chemotactic receptor. Alteration of calcium homeostasis of MβCD-treated cells does not account for the impairment of the effector responses. Disruption of microfilaments by cytochalasin B (CB) partially reverses the inhibitory effect of cholesterol depletion. Our results provide functional support for the involvement of cholesterol-rich membrane domains in the signaling of chemotactic receptors and call the attention to the possible role of microfilaments in the organization of lipid microdomains.

Journal

Biochemical and Biophysical Research CommunicationsElsevier

Published: Oct 31, 2003

References

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