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Cryptomerione induces Th1 cell polarization via influencing IL-10 production by cholera toxin-primed dendritic cells

Dendritic cells play an important role in the initiation and regulation of immune response. Dendritic cells have a key influence in the differentiation of naïve T cells into Th1, Th2 or Th17 effector cells. Cryptomerione is terpene isolated from the heartwood of Cryptomeria japonica . In this study, we investigated the effects of Cryptomerione on the phenotypic and functional maturation of human monocyte-derived dendritic cells in vitro . Human monocytes were exposed to either Cryptomerione alone, or in combination with lipopolysaccaride (LPS) or cholera toxin (CT) and thereafter co-cultured with naïve T cells. We found no enhanced CD1a, CD80, CD83, CD86 and HLA-DR expression on Cryptomerione-primed dendritic cells. However, Cryptomerione augmented T cell stimulatory capacity in an allogeneic mixed lymphocyte reaction to CT-primed dendritic cells and influenced the production of interleukin (IL)-10 and IL-12p70 by CT-primed dendritic cells, but not LPS-primed dendritic cells. Cryptomerione also inhibited Th2 cell polarization induced by CT-primed dendritic cells, but enhanced IFN-γ secretion by naïve T cells co-cultured with CT-primed dendritic cells. Cytokine production by CT-primed dendritic cells alone, or in combination with Cryptomerione was also influenced following treatment with anti-IL-10 mAb and anti-OX40L mAb. Thus, the potential mechanisms underlying the enhancement of Th1 polarization in CT-primed dendritic cells induced by Cryptomerione appeared to depend on IL-10 secretion and OX40L. These results suggest that Cryptomerione modulates human dendritic cells function in a fashion that favors Th1/Th2 cell polarization. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Pharmacology Elsevier
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