Confocal optics microscopy for biochemical and cellular high-throughput screening

Confocal optics microscopy for biochemical and cellular high-throughput screening In recent years, both academia and pharmaceutical industry have produced significant advances in confocal detection and spectroscopy by laser-induced fluorescence. Confocal fluorescence studies provide information on identity, size, diffusion coefficient and concentration of the fluorescently labeled entity. This enables the establishment of sophisticated biochemical drug screening assays using the multitude of fluorescence parameters that can be observed (e.g. molecular brightness, fluorescence lifetime, anisotropy, resonance energy transfer). In cellular screening assays, confocality introduces spatial resolution in the vertical direction and reduces background fluorescence from outside the focal plane. Confocal HTS systems focusing on femtoliter-sized observation volumes allow for assay volumes far beyond current limits. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Drug Discovery Today Elsevier

Confocal optics microscopy for biochemical and cellular high-throughput screening

Loading next page...
 
/lp/elsevier/confocal-optics-microscopy-for-biochemical-and-cellular-high-bZRirqesgr
Publisher
Elsevier
Copyright
Copyright © 2003 Elsevier Ltd
ISSN
1359-6446
D.O.I.
10.1016/S1359-6446(03)02833-2
Publisher site
See Article on Publisher Site

Abstract

In recent years, both academia and pharmaceutical industry have produced significant advances in confocal detection and spectroscopy by laser-induced fluorescence. Confocal fluorescence studies provide information on identity, size, diffusion coefficient and concentration of the fluorescently labeled entity. This enables the establishment of sophisticated biochemical drug screening assays using the multitude of fluorescence parameters that can be observed (e.g. molecular brightness, fluorescence lifetime, anisotropy, resonance energy transfer). In cellular screening assays, confocality introduces spatial resolution in the vertical direction and reduces background fluorescence from outside the focal plane. Confocal HTS systems focusing on femtoliter-sized observation volumes allow for assay volumes far beyond current limits.

Journal

Drug Discovery TodayElsevier

Published: Dec 1, 2003

References

  • Fluorescence correlation spectroscopy: lead discovery by miniaturized HTS
    Auer, M.
  • Fluorescence correlation spectroscopy. II. An experimental realization
    Magde, D.
  • Fluorescence correlations, single molecule detection and large number screening. Applications in biotechnology
    Rigler, R.
  • Fluorescence correlation spectroscopy. III. Uniform translation and laminar flow
    Magde, D.
  • Green fluorescent protein (GFP): applications in cell-based assays for drug discovery
    Kain, S.R.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off