Comparative analysis of different transformed Saccharomyces cerevisiae strains based on high-throughput Fourier transform infrared spectroscopy

Comparative analysis of different transformed Saccharomyces cerevisiae strains based on... •FT-MIR spectroscopy associated to high-throughput tool, allowed to distinguish different transformed yeast strains.•PCA allowed the analysis of the cellular metabolism related to heterologous cyprosin production based on clusters formed.•PC loadings vectors revealed the biochemical compounds differences in both expression systems.•The ratio bands can be useful to compare expression systems.•FT-MIR spectroscopy and the chemometric techniques can be used to screen and select the optimum expression system. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biotechnology Elsevier

Comparative analysis of different transformed Saccharomyces cerevisiae strains based on high-throughput Fourier transform infrared spectroscopy

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0168-1656
eISSN
1873-4863
D.O.I.
10.1016/j.jbiotec.2017.08.020
Publisher site
See Article on Publisher Site

Abstract

•FT-MIR spectroscopy associated to high-throughput tool, allowed to distinguish different transformed yeast strains.•PCA allowed the analysis of the cellular metabolism related to heterologous cyprosin production based on clusters formed.•PC loadings vectors revealed the biochemical compounds differences in both expression systems.•The ratio bands can be useful to compare expression systems.•FT-MIR spectroscopy and the chemometric techniques can be used to screen and select the optimum expression system.

Journal

Journal of BiotechnologyElsevier

Published: Oct 20, 2017

References

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