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that involves interplay between different cell types. This 3D co - culture model demonstrated that tumor -surrounding fibroblasts play important roles in distributing and connecting epithelial breast cancer cells ...
, an advanced 3D spheroid model , consisting of the luminal breast cancer MCF‑7 cells and MRC ‑5 fibroblasts , was developed. The spheroids were generated using the liquid overlay technique in culture media into 96 ...
for studying the stromal biology and for development of antitumor agents. Here, a method is described for the rapid generation of robust 3-dimensional ( 3D ) spheroid co - culture of pancreatic cancer cells ...
cells.MethodsThree different HNSCC patient-derived tumor cell lines were cultured with and without CAFs in a 3D model . Immunohistochemistry of the proliferation marker Ki67, epidermal growth factor receptor (EGFR ...
that of in the co - cultures with fibroblasts . Only, increasing the amount of fibroblasts could restore the 3D -growth. Intriguingly, co -existence of macrophage, fibroblast , and tumor cells in 3D cultures provided ...
cytotoxicity in KRASG12C-mutant spheroids when co - cultured with fibroblasts or their supernatant. The fibroblast supernatant sustained proliferative signals in tumor models . Taking into account the physical ...
are currently being developed. In this study, we established robust, reproducible, and easy-to-use short-term spheroid cultures to mimic clear cell renal cell carcinoma (ccRCC). These 3D co - cultures included ...
and 3D -2 heterogeneous cultures . Uniform conditions for 3D culturing of all three breast-cancer cell lines are proposed that results in, proliferating spheroids . When tumor cells and healthy fibroblasts ...
cell proliferation to that of a co ‐ culture model lacking this signaling. When co ‐ culture spheroids are embedded in collagen, fibroblasts producing CXCL12 promote collagen invasion of TNBC cells. Although ...
and inducing ERK1/2 activity, as shown with western blot analysis. Then, we established a stroma-rich self-assembling 3D heterospheroid model by co - culturing PANC-1 and hPSCs in 3D environment. We found ...
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