Cloning of a cDNA encoding rat aldehyde dehydrogenase with high activity for retinal oxidation

Cloning of a cDNA encoding rat aldehyde dehydrogenase with high activity for retinal oxidation Retinoic acid (RA), an important regulator of cell differentiation, is biosynthesized from retinol via retinal by a two-step oxidation process. We previously reported the purification and partial amino acid (aa) sequence of a rat kidney aldehyde dehydrogenase (ALDH) isozyme that catalyzed the oxidation of 9-cis and all- trans retinal to corresponding RA with high efficiency (Labrecque et al. Biochem. J. 305 (1995) 681–684). A rat kidney cDNA library was screened using a 291-bp PCR product generated from total kidney RNA using a pair of oligodeoxyribonucleotide primers matched with the aa sequence. The full-length rat kidney ALDH cDNA contains a 2315-bp (501 aa) open reading frame (ORF). The aa sequence of rat kidney ALDH is 89, 96 and 87% identical to that of the rat cytosolic ALDH, the mouse cytosolic ALDH and human cytosolic ALDH, respectively. Northern blot and RT-PCR-mediated analysis demonstrated that rat kidney ALDH is strongly expressed in kidney, lung, testis, intestine, stomach and trachea, but weakly in the liver. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Gene Elsevier

Cloning of a cDNA encoding rat aldehyde dehydrogenase with high activity for retinal oxidation

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Publisher
Elsevier
Copyright
Copyright © 1995 Elsevier Ltd
ISSN
0378-1119
eISSN
1879-0038
DOI
10.1016/0378-1119(96)81752-5
Publisher site
See Article on Publisher Site

Abstract

Retinoic acid (RA), an important regulator of cell differentiation, is biosynthesized from retinol via retinal by a two-step oxidation process. We previously reported the purification and partial amino acid (aa) sequence of a rat kidney aldehyde dehydrogenase (ALDH) isozyme that catalyzed the oxidation of 9-cis and all- trans retinal to corresponding RA with high efficiency (Labrecque et al. Biochem. J. 305 (1995) 681–684). A rat kidney cDNA library was screened using a 291-bp PCR product generated from total kidney RNA using a pair of oligodeoxyribonucleotide primers matched with the aa sequence. The full-length rat kidney ALDH cDNA contains a 2315-bp (501 aa) open reading frame (ORF). The aa sequence of rat kidney ALDH is 89, 96 and 87% identical to that of the rat cytosolic ALDH, the mouse cytosolic ALDH and human cytosolic ALDH, respectively. Northern blot and RT-PCR-mediated analysis demonstrated that rat kidney ALDH is strongly expressed in kidney, lung, testis, intestine, stomach and trachea, but weakly in the liver.

Journal

GeneElsevier

Published: Dec 12, 1995

References

  • Molecular Cloning
    Sambrook, J.; Fritsch, E.F.; Maniatis, T.

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