The full-length sequence of a putative Triticum aestivum serine/threonine protein kinase gene, named TaSTK , was cloned from a salt-resistant wheat line RH8706-49 based on differential expression studies of this salt-resistant line versus a salt-sensitive line H8706-34 under salt-stress conditions using cDNA-AFLP, RACE and gene amplification methods. The cDNA length is 1958 bp, of which 1431 bp form the open reading frame encoding 476 amino acids. The genomic sequence for the coding region is 4095 bp and includes 5 exons. Bioinformatic analysis suggests that the gene locates in the 2BL and 2DL chromosome and has two copies. The cDNA and genomic sequences have been submitted to GenBank database (Accession No.: AY956328 , DQ341377 ). Expression of the TaSTK mRNA was demonstrated with Northern hybridization to be enhanced under salt conditions, and the degree of salt related enhancement was greater in salt-resistant material RH8706-49 than that in the salt-sensitive material H8706-34. Excess expression of the TaSTK gene, in the form of a TaSTK gene-modified Arabidopsis thaliana material, was demonstrated to increase the growth of the roots in salt containing medium and improve the tolerance of the plant against salt stress.
Plant Science – Elsevier
Published: Jul 1, 2007
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