Cloning and characterization of two yeast genes encoding members of the CCCH class of zinc finger proteins: zinc finger-mediated impairment of cell growth

Cloning and characterization of two yeast genes encoding members of the CCCH class of zinc finger... Members of the CCCH zinc finger (Zf) protein family have in common two or more repeats of a novel Zf motif consisting of Cys and His residues in the form Cx s Cx 5 Cx 3 H (where x is a variable amino acid (aa)). We used a degenerate polymerase chain reaction (PCR) strategy to clone members of this gene family from Saccharomyces cerevisiae . The deduced aa sequences encoded by these genes, designated CTH1 and CTH2, share 46% overall identity and 59% similarity, largely due to the two highly conserved Zf domains. We found readily detectable expression of a 1.4-kb mRNA encoding Cthlp. The 1.1-kb mRNA encoding Cth2p was barely detectable under normal growth conditions; however, disruption of CTHI resulted in at least a threefold increase in CTH2 mRNA accumulation. No change in phenotype was detected following disruption of CTH1 and CTH2 , either singly or together. In contrast, overexpression of the CTH genes or one of the related mammalian genes, tris-tetraprolin ( TTP ), caused delayed entry of cell cultures into exponential growth, and a decrease in final cell density. Removal of the Zf domain of Cthlp by truncation or deletion completely reversed this slow growth phenotype, indicating that it was mediated through this highly conserved structural motif http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Gene Elsevier

Cloning and characterization of two yeast genes encoding members of the CCCH class of zinc finger proteins: zinc finger-mediated impairment of cell growth

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Publisher
Elsevier
Copyright
Copyright © 1996 Elsevier Ltd
ISSN
0378-1119
eISSN
1879-0038
D.O.I.
10.1016/0378-1119(96)00084-4
Publisher site
See Article on Publisher Site

Abstract

Members of the CCCH zinc finger (Zf) protein family have in common two or more repeats of a novel Zf motif consisting of Cys and His residues in the form Cx s Cx 5 Cx 3 H (where x is a variable amino acid (aa)). We used a degenerate polymerase chain reaction (PCR) strategy to clone members of this gene family from Saccharomyces cerevisiae . The deduced aa sequences encoded by these genes, designated CTH1 and CTH2, share 46% overall identity and 59% similarity, largely due to the two highly conserved Zf domains. We found readily detectable expression of a 1.4-kb mRNA encoding Cthlp. The 1.1-kb mRNA encoding Cth2p was barely detectable under normal growth conditions; however, disruption of CTHI resulted in at least a threefold increase in CTH2 mRNA accumulation. No change in phenotype was detected following disruption of CTH1 and CTH2 , either singly or together. In contrast, overexpression of the CTH genes or one of the related mammalian genes, tris-tetraprolin ( TTP ), caused delayed entry of cell cultures into exponential growth, and a decrease in final cell density. Removal of the Zf domain of Cthlp by truncation or deletion completely reversed this slow growth phenotype, indicating that it was mediated through this highly conserved structural motif

Journal

GeneElsevier

Published: Jan 1, 1996

References

  • Putative nuclear localization signals (NLS) in protein transcription factors
    Boulikas, T.
  • The structure of an antigenic determinant in a protein
    Wilson, I.A.; Niman, H.L.; Houghten, R.A.; Cherenson, A.R.; Connolly, M.L.; Lerner, R.A.

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