Clonal analysis of human dendritic cell progenitor using a stromal cell culture

Clonal analysis of human dendritic cell progenitor using a stromal cell culture Different dendritic cell (DC) subsets co-exist in humans and coordinate the immune response. Having a short life, DCs must be constantly replenished from their progenitors in the bone marrow through hematopoiesis. Identification of a DC-restricted progenitor in mouse has improved our understanding of how DC lineage diverges from myeloid and lymphoid lineages. However, identification of the DC-restricted progenitor in humans has not been possible because a system that simultaneously nurtures differentiation of human DCs, myeloid and lymphoid cells, is lacking. Here we report a cytokine and stromal cell culture that allows evaluation of CD34+ progenitor potential to all three DC subsets as well as other myeloid and lymphoid cells, at a single cell level. Using this system, we show that human granulocyte–macrophage progenitors are heterogeneous and contain restricted progenitors to DCs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Immunological Methods Elsevier

Clonal analysis of human dendritic cell progenitor using a stromal cell culture

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Publisher
Elsevier
Copyright
Copyright © 2015 Elsevier Ltd
ISSN
0022-1759
D.O.I.
10.1016/j.jim.2015.06.004
Publisher site
See Article on Publisher Site

Abstract

Different dendritic cell (DC) subsets co-exist in humans and coordinate the immune response. Having a short life, DCs must be constantly replenished from their progenitors in the bone marrow through hematopoiesis. Identification of a DC-restricted progenitor in mouse has improved our understanding of how DC lineage diverges from myeloid and lymphoid lineages. However, identification of the DC-restricted progenitor in humans has not been possible because a system that simultaneously nurtures differentiation of human DCs, myeloid and lymphoid cells, is lacking. Here we report a cytokine and stromal cell culture that allows evaluation of CD34+ progenitor potential to all three DC subsets as well as other myeloid and lymphoid cells, at a single cell level. Using this system, we show that human granulocyte–macrophage progenitors are heterogeneous and contain restricted progenitors to DCs.

Journal

Journal of Immunological MethodsElsevier

Published: Oct 1, 2015

References

  • Clonal type I interferon-producing and dendritic cell precursors are contained in both human lymphoid and myeloid progenitor populations
    Chicha, L.; Jarrossay, D.; Manz, M.G.
  • The origin and development of nonlymphoid tissue CD103+ DCs
    Ginhoux, F.; Liu, K.; Helft, J.; Bogunovic, M.; Greter, M.; Hashimoto, D.; Price, J.; Yin, N.; Bromberg, J.; Lira, S.A.
  • Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow
    Lee, J.; Breton, G.; Oliveira, T.Y.; Zhou, Y.J.; Aljoufi, A.; Puhr, S.; Cameron, M.J.; Sekaly, R.P.; Nussenzweig, M.C.; Liu, K.

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