Chromogenic nitrophenolate-based substrates for light-driven hybrid P450 BM3 enzyme assay

Chromogenic nitrophenolate-based substrates for light-driven hybrid P450 BM3 enzyme assay The incorporation of a p-nitrophenoxy moiety in substrates has enabled the development of colorimetric assays to rapidly screen for O-demethylation activity of P450 enzymes. For the light-driven hybrid P450 BM3 enzymes, where a Ru(II) photosensitizer powers the enzyme upon visible light irradiation, we have investigated a family of p-nitrophenoxy derivatives as useful chromogenic substrates compatible with the light-driven approach. The validation of this assay and its adaptability to a 96-well plate format will enable the screening of the next generation of hybrid P450 BM3 enzymes towards C–H bond functionalization of non-natural substrates. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Inorganic Biochemistry Elsevier

Chromogenic nitrophenolate-based substrates for light-driven hybrid P450 BM3 enzyme assay

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Publisher
Elsevier
Copyright
Copyright © 2015 Elsevier Inc.
ISSN
0162-0134
eISSN
1873-3344
D.O.I.
10.1016/j.jinorgbio.2015.12.005
Publisher site
See Article on Publisher Site

Abstract

The incorporation of a p-nitrophenoxy moiety in substrates has enabled the development of colorimetric assays to rapidly screen for O-demethylation activity of P450 enzymes. For the light-driven hybrid P450 BM3 enzymes, where a Ru(II) photosensitizer powers the enzyme upon visible light irradiation, we have investigated a family of p-nitrophenoxy derivatives as useful chromogenic substrates compatible with the light-driven approach. The validation of this assay and its adaptability to a 96-well plate format will enable the screening of the next generation of hybrid P450 BM3 enzymes towards C–H bond functionalization of non-natural substrates.

Journal

Journal of Inorganic BiochemistryElsevier

Published: May 1, 2016

References

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