Characterization of the Murine Gene Encoding Aop2 (Antioxidant Protein 2) and Identification of Two Highly Related Genes

Characterization of the Murine Gene Encoding Aop2 (Antioxidant Protein 2) and Identification of... The mouse Aop2 (antioxidant protein 2) cDNA recently cloned from liver and kidney is a member of the thiol-specific antioxidant gene family. We have isolated the mouse gene encoding Aop2 and have shown that it comprises five exons and four introns. Analysis of the sequence upstream of the translation start site revealed several potential Sp1-binding sites and two putative transcription initiation sites. Primer extension studies were used to determine the 5′ end of the Aop2 transcript. This upstream region also contains consensus recognition sequences for the transcription factors USF, SREBP, and ADR1, all of which have been shown to regulate genes involved in lipid metabolism, and multiple consensus binding sites for HSF, whose activity is modulated by oxidative stress. Since Aop2 has recently been proposed as a candidate gene for atherosclerosis susceptibility differences in mice, the presence of these binding sites may have biological significance. We also isolated two highly related intronless genes and determined their chromosomal locations. Further characterization of this highly conserved gene family and its regulation will help to elucidate their biological functions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genomics Elsevier

Characterization of the Murine Gene Encoding Aop2 (Antioxidant Protein 2) and Identification of Two Highly Related Genes

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Publisher
Elsevier
Copyright
Copyright © 1998 Academic Press
ISSN
0888-7543
eISSN
1089-8646
D.O.I.
10.1006/geno.1998.5568
Publisher site
See Article on Publisher Site

Abstract

The mouse Aop2 (antioxidant protein 2) cDNA recently cloned from liver and kidney is a member of the thiol-specific antioxidant gene family. We have isolated the mouse gene encoding Aop2 and have shown that it comprises five exons and four introns. Analysis of the sequence upstream of the translation start site revealed several potential Sp1-binding sites and two putative transcription initiation sites. Primer extension studies were used to determine the 5′ end of the Aop2 transcript. This upstream region also contains consensus recognition sequences for the transcription factors USF, SREBP, and ADR1, all of which have been shown to regulate genes involved in lipid metabolism, and multiple consensus binding sites for HSF, whose activity is modulated by oxidative stress. Since Aop2 has recently been proposed as a candidate gene for atherosclerosis susceptibility differences in mice, the presence of these binding sites may have biological significance. We also isolated two highly related intronless genes and determined their chromosomal locations. Further characterization of this highly conserved gene family and its regulation will help to elucidate their biological functions.

Journal

GenomicsElsevier

Published: Nov 15, 1998

References

  • Sequence, genomic organization, and chromosomal localization of the human LPAP (PTPRCAP) and mouse CD45-AP/LSM-1 genes
    Bruyns, E.; Mincheva, A.; Bruyns, R.M.; Kirchgessner, H.; Weitz, S.; Lichter, P.; Meuer, S.; Schraven, B.
  • LTW4 protein on mouse chromosome 1 is a member of a family of antioxidant proteins
    Iakoubova, O.A.; Pacella, L.A.; Her, H.; Beier, D.R.
  • Localization of TDPX1, a human homologue of the yeast thioredoxin-dependent peroxide reductase gene (TPX) to chromosome 13q12
    Pahl, P.; Berger, R.; Hart, I.; Chae, H.Z.; Rhee, S.G.; Patterson, D.

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